拟南芥 WRKY 基因家族应答非生物胁迫基因的鉴定1）

摘要

WRKY转录因子家族是植物特有的一类转录因子家族，在植物生长发育和应答各种胁迫反应过程中起重要作用。为筛选和鉴定植物抗逆关键基因，以拟南芥（ Arabidopsis thaliana） WRKY家族基因为研究对象，从数据库中搜索到72个拟南芥WRKY基因，通过生物信息学分析，根据其结构特点，可将其分为3大类：GroupI、GroupII和GroupIII。其中，GroupII又可分为5个亚类：IIa、IIb、IIc、IId和IIe。用实时定量RT－PCR筛选出30个应答盐胁迫的基因，其中上调表达的23个，下调表达的7个。%For screening and identifying stress-related genes in Arabidopis s thaliana, the WRKY gene families were as study ob-ject, and 72 WRKY genes were explored from the database of A.thaliana, and the structure characteristics were analyzed by bioinformatics.WRKY family was further divided into three groups:GroupI, GroupII and GroupIII.The gene members in GroupII were further divided into five sub-groups: IIa, IIb, IIc, IId, IIe.The differential expression of WRKY genes were determined by real-time RT-PCR.There were 30 genes response to salt stress, of which 23 were up-regulated genes, and 7 were down-regulated genes.