Objective To investigate the effect of the application of PBL teaching method in Medical Microbiology ex-perimental teaching. Methods 60 students from Grade 2011 majoring in inspection as the observation group were selected and adopted the PBL teaching method while another 60 students majoring in prevention of Grade 2011 as the control group were chosed to adopt LBL teaching method. The PBL teaching method effect was evaluated through their experimental skills examinations and questionnaires. Results The average score(86.65±4.49) of experimental skills examinations in the observation group was higher than that(82.05±3.72) of the control group. The differences between the two groups were statistically significant(P<0.05) Except the learning efficiency,the approval rate of the observation group in questionnaires and other items were higher than those of the control group,which had statistic significant in differences(P<0.05). Conclusion PBL teaching method may improve the teaching effect,enhance the students′practical abilities,which has been recognized widely by students.%目的：探讨基于问题的学习（PBL）教学法在《医学微生物学》实验教学中的应用效果。方法选取新乡医学院2011级检验专业60名学生为观察组，采用PBL教学法；另选取新乡医学院2011级预防专业60名学生为对照组，采用传统基于课堂的学习（LBL）教学法，通过实践技能考试及问卷调查方式综合评价PBL教学法的教学效果。结果观察组学生实验技能考核成绩平均得分[（86.65±4.49）分]高于对照组[（82.05±3.72）分]，差异有统计学意义（t=3.529，P=0.001）。除提高学习效率一项外，观察组学生对于调查问卷其他项赞成率明显高于对照组，差异均有统计学意义（P＜0.01）。结论 PBL教学法提高了教学效果，提升了学生实践操作能力，得到了学生的广泛认同。
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