中国汉族2型糖尿病患者血管紧张素原基因单核苷酸多态性分析研究

摘要

Objective To investigate the association between five single nucleotide polymorphisms (SNP) in angiotensinoge (AGT) gene with type 2 diabetes mellitus(T2DM) in Chinese Han nationality. Methods rs3889728 ,rs2478523 ,rsl926722 , rs7539020 and rs2493132 of AGT gene were analyzed with allele-specific fluorogenic oligonucleotide probes in an assay combining extension and hybridization. Results In rs2478523, the frequency of CT genotype (0. 382 vs 0. 505, Z= 5. 664, P = 0. 017) was lower and TT genotype (0. 435 vs 0. 307, Z=6. 389, P=0. 012) was higher in T2DM group than control group. The frequencies of the other 4 SNPs' genotypes and all 5 SNPs' alleles were not significantly different between the two groups (Z=0. 001～ 3. 041, all P>0. 05). According the level of linkage disequilibrium, the frequencies of the haplotypes constructed with rs3889728, rsl926722 and rs7539020 were not significantly different between the two groups (all P> 0. 05). Conclusion Polymorphism of rs2478523 in AGT gene may be a genetic susceptibility factor for T2DM in Chinese Han nationality.%目的 观察中国汉族人群血管紧张素原(AGT)基因5个单核苷酸多态性(SNP)与2型糖尿病(T2DM)的相关性.方法 应用荧光标记单碱基延伸及寡核苷酸微阵列芯片杂交技术检测AGT基因rs3889728,rs2478523,rs1926722,rs7539020和rs2493132的基因型.结果 T2DM组rs2478523的CT基因型频率(0.382 vs 0.505)显著低于对照组(Z=5.664,P=0.017),而TT型频率(0.435 vs 0.307)显著高于对照组(Z=6.389,P=0.012).余4个SNP的基因型和5个SNP的等位基因频率两组间差异无统计学意义(Z=0.001～3.041,P值均>0.05).根据连锁不平衡水平,由rs3889728,rs1926722和rs7539020构建的单倍型频率在两组间差异无统计学意义(P值均>0.05).结论 AGT基因的rs2478523多态性可能是中国汉族人群T2DM的遗传易感因素.