培养小鼠髓系DC2.4细胞,加入LPS(阳性对照组)或甘草甜素,用扫描电镜观察DC的超微结构、流式细胞仪检测DC表面分子MHCⅡ、CD86及CD40的表达、4,氨基安替比林(4-AAP)比色检测DC内酸性磷酸酶活性、ELISA方法检测DC培养上清中IL-12的浓度,体外刺激淋巴细胞增殖实验检测DC对同种异体T淋巴细胞的刺激能力.结果表明,与对照组相比,甘草甜素刺激后,DC表面树突状突起增多,表面分子MHCⅡ、CD86及CD40表达增加,酸性磷酸酶活性下降,培养上清中IL-12浓度升高,刺激同种异体T淋巴细胞的能力也明显增强.结果表明,甘草甜素能够促进小鼠髓系DC2.4表型及功能的成熟.%Myelogenous cells of mice DC2.4 were cultured and added with LPS (positive control group) or glycyrrhi-zin (GL) , and observed with scanning electron microscope (SEM) for the DC ultra micro-structure; with flow cytom-eter to test the expression of surface molecules MHC II, CD86, and CD40; with 4-amino-antipyrin (4-AAP) chro-mometry to test the activity of acidic phosphatase inside the DC; with ELISA to test the concentration of IL-12 in the cultural supernatant of DC; with T-cell proliferation experiment stimulated in vitro to test the stimulation capability against allogeneic T-cell. The results showed that as compared with the control group, after stimulated by GL, the protuberance of DC surface increased, the expression of surface molecules MHC II, CD86, and CD40 increased, the activity of acidic phosphatase decreased, the concentration of IL-12 in cultural supernatant rose, and the capability of cells to stimulate against allogenic T-cell obviously strengthened. Therefore, it could be concluded that GL could enhance the phenotype and function of myelogenous cells of mice DC2.4 to mature.
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