异源副粘病毒感染对雏鸭血清抗氧化酶活性的影响

摘要

Ducklings were taken as experimental objects to study the effects of different sources of paramyxovirus on ducklings′serum antioxidase activities. 100 healthy ducklings in the age of 20 days were randomly selected and ran-domly divided into group I,II,III,and IV,group I was taken as control group,and subcutaneous injected with 0.5 ml/duckling saline,group II,III,and IV were respectively and subcutaneously injected 0.5 mL/duckling with chick-en source,goose source and duck source paramyxovirus SPF embryo fluid. And respectively at 7,14,21,and 28 d after the viral attack,5 ducklings from each group randomly selected were carried out their blood collection and serum separation,the activities and contents of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde(MDA) in ducklings′serum were determined,to study the effect of heterologous pa-ramyxovirus infection on the ducklings′antioxidase activity,and the degree of lipid peroxidation and changing trend of antioxidase activity. The results showed that the activities of SOD,CAT,GSH-Px and MDA in the ducklings′ serum of infected with heterologous paramyxovirus after the viral attack were significantly different within different time, re-vealed that free radical metabolism of ducklings′ serum produced different degree of effects. Therefore, heterologous paramyxovirus had different effects on the free radical metabolism of ducklings, and the degree of lipid peroxidation was closely related to SOD and GSH-Px activities.%以雏鸭为试验对象,研究不同来源的副粘病毒感染对雏鸭血清中抗氧化酶活性的影响.选择20日龄健康雏鸭100只,随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ组,Ⅰ组为对照组,皮下注射0.5 mL/只 生理盐水,Ⅱ、Ⅲ、Ⅳ组分别皮下注射0.5 mL/只稀释的鸡源、鹅源及鸭源副粘病毒SPF胚液.分别在攻毒后7、14、21、28 d时,随机抽取Ⅰ、Ⅱ、Ⅲ、Ⅳ组雏鸭各5只进行血液采集与血清分离,测定雏鸭血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的活性与含量,研究异源副粘病毒感染对雏鸭血清中抗氧化酶活性的影响,以及脂质过氧化程度与抗氧化酶活性的变化趋势.结果表明,在攻毒后不同时间内,感染异源副粘病毒的雏鸭血清中的SOD、CAT、GSH-Px活性和MDA活性与含量变化显著不同,揭示异源副粘病毒对雏鸭血清自由基代谢产生了不同程度影响,脂质过氧化程度与SOD、GSH-Px活性密切相关.