Objective To explore the test - retest reliability of aggressive behavior test parameters, as a method for evaluating ethology in anger emotion rats. Methods With resident intruder and social isolation stress, typical anger emotion in 40 male Wister rats was produled. The following parameters were evaluated: latent time, number of attacks, attack durations, number of bites, on - top durations and scores of composite aggression. The intractass correlation coefficient (ICC) and the agreement index ( Kappa) were calculated for each of these parameters to explore the test - retest reliability of the above parameters of aggressive behavior. Results Both ICC and Kappa for composite aggression ( Kappa =0.662,P <0.001; ICC =0.910,P <0.001) showed a good reliability between the test parameters. Attack durations ( Kappa =0.533 ,P<0.001; ICC =0. 898,P <0.001) showed a fair reliability between the test parameters. Only ICC for number of attack (ICC =0. 834,P <0.001)and latent lime(ICC =0.671 ,P <0.001)showed a good reliability between the test parameters. Neither ICC nor Kappa displayed a significant reliability between the rest parameters. Conclusion The score of composite aggression , which had a good lest - retest reliability, was found to be a stable parameter in aggressive behavior of anger emotion rats. The aggressive behavior test of rats with anger emotion was also affected by both environmental factors and the isoforms of anger emotion.%目的 探讨攻击行为测试参数作为怒情绪大鼠行为学评价方法的复测信度.方法 40只雄性Wistar大鼠,应用居住入侵配合社会隔离慢性应激造成大鼠出现典型“怒”情绪改变.通过组内相关系数(ICC)和一致性系数(Kappa)对实验进行复测信度检验,参数如下:潜伏期( latent time)、攻击次数(number of attacks)、攻击持续时间(attack durations)、撕咬次数(numberof bites)、攀压持续时间(on - top durations)、混和攻击行为得分(composite aggression).结果 ①Kappa结果表明,仅混和攻击行为得分( Kappa=0.662,P＜0.001)具有高度的可信度,攻击持续时间(Kappa=0.533,P＜0.001)具有中等的可信度,其余参数可信度较弱；②ICC结果表明,攻击次数(ICC=0.834,P＜0.001),攻击持续时间(ICC=0.898,P＜0.001)、混和攻击行为得分(ICC=0.910,P＜0.001)具有极高的可信度,潜伏期(ICC =0.671,P＜0.001)具有高度的可信度,其余参数可信度一般.结论 混和攻击行为得分可以作为怒情绪大鼠攻击行为评价的稳定参数,具有较好的复测信度；怒情绪大鼠攻击行为结果亦受环境因素和怒情绪本身亚型分类的影响.
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