目的:探讨高架十字迷宫(elevated plus-maze,EPM)作为焦虑大鼠模型评价方法的复测信度及其参数相关性.方法:将成年雌性Wistar大鼠放入高架十字迷宫中,摄像系统记录5 min的行为变化,实验间隔1周进行2次;实验参数如下:进入开放臂次数、进入封闭臂次数、向下探究次数、封闭臂后腿直立次数、进入开放臂时间、进入封闭臂时间.结果:①组内相关系数(ICC)结果表明,除封闭臂后腿直立次数和向下探究次数初测与复测相关性差外,其他参数均显示EPM的初测与复测具有较好的相关性.②一致性系数(Kappa)结果表明,所有参数均显示EPM复测可信度较好,Kappa值均＞0.5.结论:进入开放壁时间百分比、进入开放壁次数百分比和总进臂次数在EPM初测及复测实验中均呈较好复测信度.%Objective: To investigate the retest reliability and parameter correlation of the elevated plus-maze (EPM) experiment as the evaluation method of anxious rat model. Methods: The camera system recorded the behavioral changes within 5 minutes of adult female Wistar rats placed in the elevated plus-maze twice every other week. The experimental parame ters were as follows: times of entering the open arm, times of entering the closed arm, times of downward exploration, times of rear leg upright standing in the closed arm, time into the open arm and time into the closed arm. Results: ①The ICC results showed that all the other parameters demonstrated good correlation of EPM between the initial test and the retest ex cept the poor correlation of times of rear leg upright standing in the closed arm and times of downward exploration between the initial test and the retest. ②The Kappa results showed that all the parameters demonstrated good reliability of EPM retest, with all Kappa values higher than 0.5. Conclusion: The percentage of time into the open arm, the percentage of times of entering the open arm and the total times of entering arms show good retest reliability in both EPM initial test and retest experiments.
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