稳定表达DLC-1的淋巴瘤细胞株模型的建立

摘要

Objective To establish a human lymphoma cell line that stably expresses deleted in human liver cancer-1 gene(DLC-1).Methods The reconstructed plasmid,pcDNA3.1-DLC-1,was transfected to the human lymphoma cell line of Raji.Then the transfected Raji cells were selected by G418.The stable overexpression of DLC-1 was identified by Western blot assay.Results The recombined plasmid,pcDNA3.1-DLC-1,was confirmed by restriction endonuclease examination and sequencing.Western-blot showed that DLC-1 protein expression was increased in the transfected Raji cells.Conclusion A human lymphoma cell line with stable expression of DLC-1 is established.%目的 建立稳定表达肝癌缺失基因-1(DLC-1)的淋巴瘤细胞株.方法 将DLC-1真核表达质粒转染人淋巴瘤细胞株Raji,G418筛选,对获得的Raji细胞株进行Western blot鉴定.结果 酶切和测序鉴定DLC-1的cDNA片段正确插入pcDNA3.1质粒,Western blot鉴定转染后Raji细胞株DLC-1高表达.结论 成功建立了稳定高表达DLC-1的淋巴瘤细胞株.