RNA干扰TGF-β信号通路对人膀胱癌T24细胞迁移和侵袭能力的影响

摘要

Objective To evaluate the effects of TGF-β1 and TsiRNA targets the TGF-β type Ⅰ receptor on the motility and invasiveness of T24 cell.Methods We blocked the TGF-β signal pathway in T24 human bladder cancer cells with a siRNA (TsiRNA)which targets the TGF-β type Ⅰ receptor and evaluated the effects of TGF-β1 and TsiRNA on the cell motility and invasiveness by Matrigel migration assay,wound-healing assay and Matrigel invasion assay.Western blot and RT-PCR analysis were used to examine the effects of TGF-β1 and TsiRNA on the expression of TGFBRI.Results Our study revealed an addition of TGF-β1 (2 ng/ml) significantly enhanced the migration and migration of the T24,while this enhanced ability could be effectively inhibited by TsiRNA.Conclusion This study suggested that TGF-β1 was a major enhancer for the motility and invasiveness of bladder cancer cells.The TGFBRI targeted siRNA strongly inhibited the motility and invasiveness of the bladder cancer cells.%目的 探讨过表达的TGF-β1和作用于TGF-β受体Ⅰ的干扰RNA(TsiRNA)在体外对人膀胱癌T24细胞迁移、侵袭能力的影响.方法 采用Transwell迁移试验、划痕试验以及Transwell侵袭实验观察过表达的TGF-β1和TsiRNA在体外对人膀胱癌T24细胞迁移、侵袭能力的影响,RT-PCR技术和蛋白免疫印迹法(Western blot)检测TGF-β1及TsiRNA处理后TGF-β受体Ⅰ基因与蛋白表达水平的变化.结果 过表达的TGF-β1可以显著提高膀胱癌T24细胞的迁移、侵袭能力,而TsiRNA可以完全降低由TGF-β1引起的T24的迁移、侵袭能力的变化.结论 膀胱癌细胞的迁移、侵袭能力与过表达的TGF-β1密切相关,TsiRNA可以降低T24细胞的迁移、侵袭能力.