用傅立叶变换红外光谱法研究核辐照对虫草粉成分的影响

摘要

本文采用傅立叶变换红外光谱(卷积谱及二阶导数谱)法对经不同剂量核辐照的虫草粉进行了对比研究.虫草粉中碳水化合物(糖和纤维素)、蛋白质及脂肪含量较高；虫草的多糖及酯对核辐照较为敏感,辐照还使分子中吡喃环的C-O-H基团向多糖的O-H和酯的C-O-C基团转化;构成虫草蛋白质的多肽是α构象的；低于18 kGy剂量辐照虫草粉样品对虫草蛋白质影响很小,但对虫草主要有效成分(含有C-N基团和N-H基团成分)影响较大；高于18 kGy剂量辐照虫草粉样品对虫草蛋白质、多糖及酯的分子结构可能产生了破坏.建议不宜采用核辐照对虫草粉杀菌消毒处理.%FTIR spectra of Cordyceps sinensis (Berk.) Sacc powder irradiated by y-ray with different doses were studied in the paper . Meanwhile, Fourier self-deconvolution curves and the second derivative specra for the irradiated Cordyceps sinensis (Berk. ) Sacc powder were also studied. The content of carbohydrate(sugar and cellulose) . protein and fat are higher in the Cordyceps sinensis (Berk. ) Sacc powder; y-ray irradiation for amy-lose and ester of Cordyceps sinensis (Berk.) Sacc powder is sensitiver, y-ray irradiation for Cordyceps sinensis (Berk. ) Sacc powder makes the group (C-O- H) of pyran ring of molecule toward the group (O-H) of sugar and the group (C-O-C) of ester; The poly-peptide that constitute protein of Cordyceps sinensis (Berk. ) Sacc are many α -configuration; It was found that the protein of the irradiated Cordyceps sinensis (Berk. ) Sacc powder with the under 18 kGy had almost no changes, however, the influence of the mainly effective composition (composition of group C-N and group N-H) of Cordyceps sinensis (Berk. ) Sacc powder is larger . The molecular structure of protein, amylose and cellulose of the irradiated Cordyceps sinensis (Berk. ) Sacc powder with the dose of beyond 18 kGy could be broken. It is suggested that the sterilization and disinfection of Cordyceps sinensis (Berk. ) Sacc powder could not use the y-ray irradiation.