树舌荧光多糖的制备及其在人大肠癌细胞SWWC1116中的定位

摘要

The reductive amination method was used for the fluorescently labelling of Ganoderma applanatum polysaccharide(GAP). FITC was linked up with the reducibility termination of GAP, and fluorescent substitute ratio was determined to be 0. 90%. The results of the measurement by xCELLigence RTCA DP automatic real-time cell analyzer show in vitro the cytotoxic activity did not significantly change before and after fluorescent labeling of GAP. The results measured by means of flow cytometer and fluorescence microscope show fluorescence signal was detected both-on the cell membrane and in the nucleus, illustrating that GAP can be attached to human colorectal cancer SWWC1116 membrane, and can be further transported to the nuclei.%应用胺化还原法在树舌灵芝液体深层发酵浸膏水提多糖(GAP)的还原性末端连接荧光基团(FITC),制备荧光标记GAP (FITC-GAP),并测定其荧光取代率为0.90％.利用xCELLigence RTCA DP全自动实时细胞分析仪检测GAP荧光标记,其前后的细胞毒活性不变.流式细胞仪和荧光显微镜对GAP在人大肠癌细胞SWWC1116定位结果表明,GAP可与人大肠癌细胞SWWC1116的细胞膜结合,并可转运至细胞核内.