菩人丹超微粉对糖尿病大鼠视网膜血流动力学和细胞因子表达的影响

摘要

Objective:To investigate the effects of Purendan Superfine Powder (PRD) on the retinal hemodynamics and the expressions of cytokines in the diabetic rats, and to elucidate its protective effect on diabetic retinopathy (DR) .Methods:Thirty-six male SD rats were randomly divided into control group, diabetes group, and PRD group (n=12) .The diabetes rat models were established by intraperitoneal injecting streptozotocin (STZ) in diabetes group and PRD group.After successful modeling, the diabetic rats in PRD group were given PRD (1.8g·kg-1) by gavage for 3months.The hemodynamics parameters of central retinal artery (CRA) of the rats were measured with color Doppler ultrasound.The expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNA in retina tissue were detected by reverse transcription polymerase chain reaction (RT-PCR) .Immunohistochemical staining and Western blotting methods were used to detect the expression levels of VEGF and bFGF proteins in retina tissue of the rats.Results:Compared with control group, the blood glucose, urine volume, liver index and kidney index of the rats in diabetes group were significantly increased (P＜0.05) , the peak systolic velocity (PSV) , end diastolic velocity (EDV) and mean velocity (MV) of CRA were significantly decreased (P＜0.05) , the resistant index (RI) and pulsatility index (PI) of CRA were significantly increased (P＜0.05) , and the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly increased (P＜0.05) .Compared with diabetes group, the blood glucose, urine volume, liver index and kidney index of the rats in PRD group were significantly decreased (P＜0.05) , the PSV, EDV and MV of CRA were increased (P＜0.05) , the RI and PI of CRA were decreased (P＜0.05) , the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly reduced (P＜0.05) .Conclusion:PRD can protect the diabetic retinal microvascular injury by reducing the blood glucose level, increasing the blood supply of retinal tissue, increasing the amount of microcirculation blood perfusion, reducing the vascular bed perfusion resistance, and reducing the expression of VEGF and bFGF.%目的:探讨菩人丹超微粉 (简称PRD) 对糖尿病大鼠视网膜血流动力学和细胞因子表达的影响, 阐明PRD对糖尿病视网膜病变 (DR) 的保护作用.方法:36只雄性SD大鼠随机分为对照组、糖尿病组和PRD组, 每组12只.糖尿病组和PRD组大鼠腹腔内注射链脲佐菌素 (STZ) 建立糖尿病模型.建模成功后, PRD组大鼠灌胃给予PRD (1.8g·kg-1) , 连续3个月.采用多普勒超声检测大鼠视网膜中央动脉 (CRA) 血流动力学参数, 采用逆转录聚合酶链反应 (RT-PCR) 检测视网膜组织中血管内皮生长因子 (VEGF) 和碱性成纤维细胞生长因子 (bFGF) mRNA表达水平, 采用免疫组织化学染色法和Western blotting法检测视网膜组织中VEGF和bFGF蛋白表达水平.结果:与对照组比较, 糖尿病组大鼠血糖、尿量、肝指数和肾指数明显升高 (P＜0.05) , 视网膜CRA峰血流速度 (PSV) 、舒张期末流速 (EDV) 和平均血流速度 (MV) 明显降低 (P＜0.05) , 阻力指数 (RI) 和搏动指数 (PI) 明显升高 (P＜0.05) , 视网膜组织中VEGF和bFGF mRNA及蛋白表达水平明显升高 (P＜0.05) .与糖尿病组比较, PRD组大鼠血糖、尿量、肝指数和肾指数明显降低 (P＜0.05) , 视网膜CRA的PSV、EDV和MV明显升高 (P＜0.05) , RI和PI明显降低 (P＜0.05) , 视网膜组织中VEGF和bFGF mRNA及蛋白表达水平明显降低 (P＜0.05) .结论:PRD可降低糖尿病大鼠血糖水平, 增加视网膜组织血供, 增加微循环血液灌注量, 降低血管床灌注阻力, 下调VEGF与bFGF表达, 对糖尿病视网膜微血管损伤起保护作用.