目的：探索苯并芘[Benzo（a）pyrene，BaP]是否对人早孕胎盘绒毛膜外滋养层细胞HTR-8/SVneo具有毒性作用以及五味子乙素（Schisandrin B，Sch B）是否可以降低BaP的毒性作用。方法：以HTR-8/SVneo细胞为载体，构建BaP对HTR-8/SVneo细胞的染毒模型和Sch B对HTR-8/SVneo细胞的保护模型，利用MTS细胞增殖实验检测不同浓度的BaP单独给药和Sch B联合BaP给药对HTR-8/SVneo细胞增殖的影响。结果：0.01，0.1μmol/L浓度的BaP并不抑制HTR-8/SVneo细胞的增殖（P＞0.05），而1，10，100μmol/L浓度的BaP对HTR-8/SVneo细胞的增殖有抑制作用（P＜0.05）且随着其作用时间（6，12，24，36和48 h）的延长而增大，但作用24 h后其抑制作用趋于平衡，通过绘制HTR-8/SVneo细胞的生长曲线发现，20μmol/L的BaP对HTR-8/SVneo细胞作用24 h后的抑制作用最佳，其抑制率达到24.95%；0.01，0.1，1μmol/L浓度的Sch B并未抑制HTR-8/SVneo细胞的增殖（P＞0.05），高于10μmol/L的Sch B抑制HTR-8/SVneo细胞的增殖（P＜0.05）。0.25，0.5，1，2μmol/L的Sch B可以降低BaP的毒性作用且对HTR-8/SVneo细胞没有毒性作用，且其保护作用随着浓度增加而增大（P＜0.05），其中2μmol/L的Sch B对HTR-8/SVneo细胞的保护作用最强，保护率达到17.84%。结论：BaP对HTR-8/SVneo细胞具有毒性作用，而一定浓度范围内的Sch B可以有效地降低BaP的这种毒性作用。%Objective:To investigate whether administering of benzo(a)pyrene (BaP) and schisandrin B (Sch B) to human trophoblast HTR-8/SVneo cells has cytotoxicity and cytoprotection, respectively. Methods:By HTR-8/SVneo cells culture in vitro, MTS cell proliferation assay was performed to detect the effect of different concentration of BaP-alone, Sch B-alone and Sch B combining with BaP treatment on cell proliferation. Results:0.01 and 0.1μmol/L BaP did not inhibit HTR-8/SVneo cell proliferation (P>0.05), but 1, 10 and 100μmol/L BaP all inhibited HTR-8/SVneo cell proliferation (P<0.05). The inhibition rate were presented to be escalated accompanied by escalating BaP time exposure (6, 12, 24, 36 and 48 h), and 24 h BaP exposure were presented to be a saturation, especially for 20 μmol/L BaP with 24 h exposure which presented a suitable inhibition rate (24.95%). Besides, 0.01, 0.1 and 1μmol/L Sch B did not inhibit HTR-8/SVneo cell proliferation (P>0.05), but the level of SchB higher than 10μmol/L inhibited HTR-8/SVneo cell proliferation (P<0.05). 0.25, 0.5, 1 and 2μmol/L Sch B all attenuated the cytotoxicity induced by BaP in HTR-8/SVneo cells with a dose-effect relationship, especially for 2 μmol/L Sch B which presented a highest protection rate (17.84%). Conclusions:BaP can directly induce cytotoxicity in HTR-8/SVneo cells, while certain Sch B can attenuate the cytotoxicity.
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