超高效液相色谱-串联质谱法同时检测植物叶片中吲哚-3-乙酸及其3种氧化产物

摘要

以玉米叶片为供试材料,建立了同时测定植物体内吲哚-3-乙酸(IAA)及其3种氧化产物吲哚-3-甲醇(ICI)、吲哚-3-甲醛(ICA)、吲哚-3-羧酸(IFA)含量的超高效液相色谱-串联质谱(UPLC-MS/MS)方法.结果表明,该方法对IAA及其3种氧化产物检测的线性、精密度和重复性较好,灵敏度较高,4种化合物的检出限为0.002 ～1.63 μg/kg,定量下限为0.007 ～5.43 μg/kg;方法的加标回收率为89.5％ ～95.3％,相对标准偏差为2.3％ ～5.1％.玉米叶片的实际测定结果表明,IAA,ICI,ICA和IFA的含量分别为(196.25±7.10),(26.21±2.13),(18.65±2.02),(13.62±2.06) μg/kg.该方法已成功应用于小麦、豌豆、硬毛刺苞菊叶片的测定,通用性较好.%An ultra-performance liquid chromatography-tandem mass spectrometric (UPLC-MS/ MS) method was developed for the simultaneous determination of indole-3-acetic acid (IAA) and its three oxidative products,indole-3-methanol(ICI),indole-3-carboxaldehyde (ICA) and indole-3-carboxylic acid(IFA) in corn(Zea mays L.) leaves.The results showed that the detection limits for the four compounds were in the range of 0.002-1.63.μg/kg,and the quantitation limits were 0.007-5.43 μg/kg.The recoveries ranged from 89.5％ to 95.3％ with RSDs of 2.3％-5.1％.The mthod was applied in the determination of the contents of IAA,ICI,ICA and IFA in corn leaves with their results of (196.25 ±7.10),(26.21 ±2.13),(18.65 ±2.02),(13.62 ±2.06) μg/kg,respectively.Meanwhile,the proposed method was also successfully applied in the analysis of wheat (Triticum aestivum L.),pea (Pisum sativum L.) and bristly starbur (Acanthospermum hispidum DC.),which indicated that the method had a good generality.