仔猪水肿病多价油乳剂灭活菌苗在猪体内的免疫原性研究

摘要

用自制的仔猪水肿病多价油乳剂灭活菌苗 肌肉注射20日龄未接种同类疫苗的长杂健康仔猪，14 d后用攻毒菌株（ 湖北地方分离株及O 138、O139、O141 3个菌株）攻毒。结果表明每头免疫剂量为0.50 mL ( 含30亿CFU)免疫效果最好，免疫2周后血清抗体几何凝集效价为1∶640，保护率达到100%。 %Intramuscular injection of 20-day-old healthy Chan gza piglets without inoculating similar vaccine with piglet oedema disease multi valent oil-emulsion inactivated vaccine was carried out.the piglets were cha llenged with Hubei local isolated strains and O138，O139 and O14 1.Results showed that 0.50　mL(containing 30×108　CFU) immune dose per pigl et had the best effect, and two weeks later the piglets' serum antibody geometric agglutination titer reached 1∶640, protection rate reached 100%.