硫酸化茯苓多糖对MPP+诱导的PC12细胞损伤的保护作用研究

摘要

In order to explore the protective effect of sulfation pachymaran on the damage of PC12 cells in⁃duced by MPP+,PC12cells were co-cultured with different concentrations of SP to determine its potential tox⁃icity;Using MPP+ induced PC12 cells as the cell model of Parkinsons disease,the cell viability was detected by MTT assay.Morphological changes were observed under microscopy,and the contents of SOD,GSH-Px, LDH,and MDA were tested by UV spectrophotometer.The result showed that no potential cell toxicities of SP in the range of 0.5~4 mg/mL were observed;PC12 cells treated with 200 umol/L MPP+ for 24 h were used as damage and model;the number of PC12 cells in MPP+ induced model group obviously decreased;cell pro⁃cesses shrunk or vanished,cell bodies became smaller,meanwhile the contents of LDH and MDA were signif⁃icantly increased (all P<0.01),while the activities of SOD and GSH-Px were markedly decreased (all P<0.01). Compared with the model group,by treatment with 2~4 mg/mL SP,cell viability was significantly in⁃creased (P<0.05),the number and volume of cells were also increased,and the adherent abilities of cells were recovered obviously.The levels of LDH and MDA were significantly decreased (all P<0.01),whereas the activities of SOD and GSH-Px were markedly increased (all P<0.01). SP at certain concentrations may inhibit MPP+induced oxidative stress damage in PC12 cells.%为探究硫酸化茯苓多糖（SP）对MPP+损伤的PC12细胞的保护作用，通过一定浓度的SP与PC12细胞共培养，检测SP的毒性作用；以MPP+损伤PC12细胞作为帕金森病（PD）细胞模型，MTT法检测细胞活力，显微镜观察细胞形态，紫外分光光度计法测定SOD、GSH-Px、LDH活性以及MDA含量.结果表明：SP浓度在0.5~4 mg/mL范围内对PC12细胞没有毒性；200μmol/L MPP+作用于PC12细胞24 h建立损伤模型；MPP+模型组细胞数量明显减少，细胞突起收回或消失，胞体变小、变圆；LDH释放量、MDA含量显著增加（均为P<0.01），SOD和GSH-Px活性极显著降低（均为P<0.01）.与模型组相比，SP浓度在2~4 mg/mL时，细胞活力增大（P<0.05），细胞数量显著上升，胞体变大，贴壁能力恢复明显；LDH的释放量、MDA含量显著下降（均为P<0.01），SOD和GSH-Px活性显著提高（均为P<0.01）.一定浓度的SP能够抑制MPP+对PC12细胞的氧化应激损伤.