酵母SOD1基因缺失突变体应答真菌细胞壁抑制剂CFW的转录组学分析

摘要

This paper investigated the effect of SOD1 gene deletion on the genome-wide transcriptional re-sponse to fungal cell wall-perturbing agent Calcofluor white( CFW) in Saccharomyces cerevisiae using DNA microarray,to provide a new theoretical basis for revealing the regulatory mechanism of cell walls of plant pathogenic fungi and optimizing plant anti-fungal genetic engineering. The results showed that 211 genes (97 up-regulated genes and 114 down-regulated genes) were differentially expressed in sod1Δyeast cells compared with the wild type yeast cells after CFW treatment ( 10 μg/mL for 1 h ) . The microarray data were further confirmed by qPCR analysis,using five randomly selected,differentially expressed genes. The differentially expressed genes were mainly related to cell wall,cell metabolism,protein synthesis,cell de-fence,and included genes with unknown functions. Taken together,SOD1 deletion significantly affects the genome-wide transcriptional profile in response to the cell wall-perturing agent CFW in yeast.%以酿酒酵母为研究材料,采用酵母全基因组表达谱芯片,分析超氧化物歧化酶SOD1基因缺失( sod1Δ)对酵母细胞应答真菌细胞壁抑制剂钙荧光白( CFW)全基因组转录表达谱的影响,为揭示植物病原真菌细胞壁调控机制以及植物抗真菌基因工程改造提供新的理论基础. 结果表明:CFW (10 μg/mL)处理1 h后,与野生型酵母细胞相比,sod1Δ酵母细胞中211个基因发生了显著差异表达(97个基因表达上调、114个基因表达下调). 随机选取5个差异表达基因采用定量PCR验证,结果与芯片分析结果一致. 差异表达基因功能主要涉及细胞壁、细胞代谢、蛋白质合成、细胞防御以及大量功能未知蛋白. 以上结果表明, SOD1 基因缺失可显著改变酵母细胞应答真菌细胞壁抑制剂CFW胁迫的全基因组转录表达谱.