白术内酯Ⅰ对慢性萎缩性胃炎大鼠胃黏膜保护作用

摘要

Objective: to explore the protective mechanism of Atractylenolide I for gastric mucosa of chronic atrophic gastritis ( GAC) rats.Methods: The laboratory animals were randomly divided into control group, model group and Atractylenolide I group (10, 20, 40 mg/kg) .The models were established by alternate intragastric administration with sodium deoxycholate and hot saline, which was combined with irregular diet.8 weeks later, ELISA was taken to detect IL-8 content, and Western blot to detect HSP 70, NF-κB and COX-2 expressions.Results:Compared to control group, HSP 70 of model group decreased ( P <0.01), IL-8 increased ( P <0.01), , NF-κB and COX-2 increased ( P <0.01);compared to model group, low, medium and high dose groups of Atracty-lenolide I increased HSP 70 expression, decreased IL-8, NF-κB and COX-2 ( P <0.05), especially significantly in the high dose group (40 mg/kg) .Conclusion:Atractylenolide I may provide protection for CAG rats through anti-inflammatory action.%目的：探讨白术内酯Ⅰ对慢性萎缩性胃炎（ CAG）大鼠胃黏膜保护机制。方法：实验动物随机分为空白对照组、模型组、白术内酯Ⅰ组（10、20、40 mg／kg），采用脱氧胆酸钠和热盐水交替灌胃、结合饥饱失常方法制备CAG大鼠模型，治疗8周后，采用ELISA法检测白细胞介素－8（IL－8）含量、 Western blot法检测热休克蛋白70（HSP 70）、核转录因子－k B （NF－kB）和环氧合酶－2（COX－2）蛋白表达。结果：与空白对照组对比，模型组HSP70蛋白表达降低（ P ＜0．01）， IL－8含量（ P ＜0．01）增加、 NF－κB和COX－2蛋白表达升高（ P＜0．01）；与模型组对比，白术内酯Ⅰ高、中、低3个剂量组提高HSP70蛋白表达，降低IL－8含量、 NF－κB和COX－2蛋白表达（ P ＜0．05），以白术内酯Ⅰ组（40 mg／kg）作用显著。结论：白术内酯Ⅰ对CAG大鼠的保护作用是通过抗炎作用。