Objective: To study the effect and the therapeutic mechanism of Kui Jie Shuang enema on experimental ulcerative coli-tis (UC) rats from the angle of oxidative stress and energy metabolism, and to analyze the effect of the single factor effect and the dose-effect relationship on the UC rats at different levels by orthogonal design. Methods: according to L9 (34) orthogonal design, 70 clean male Wistar rats were tested in 9 groups. After replicating the rat model of ulcerative colitis with dextran sodium sulfate (DSS) meth-od, Kui Jie Shuang was used for enema for 10 d; materials was selected by anesthesia, the colonic mucosa injury score (CMDI) was calculated, and the pathological sections were made. The biochemical method was used to detect the glutathione (GSH), the activity of superoxide dismutase (SOD), and the expression levels of malondialdehyde (MDA), adenosine triphosphate (ATP) and nitric oxide (NO) in the rat's colon mucosa. Results: the activity of antioxidant factor SOD and GSH decreased significantly in the model group, the level of MDA and NO increased significantly, the energy metabolism was influenced, the level of ATP decreased, and the differ-ence was significant ( P <0. 05). Kui Jie Shuang enema could improve the oxidative stress injury and energy metabolism of UC rats in different degrees, improve the activity of SOD and GSH and the level of ATP, and reduce the expression of MDA and NO, with the best effect in group B ( P <0. 05). Compared to model group, CMDI in group A, B, C, E, F and I had different degrees of improvement ( P <0. 05), and the result was consistent with the changes of expression level of activity of SOD and GSH and energy ATP expres-sion. The 4 Chinese herbs, Huangbai, Kushen, Baiji and Diyu of different doses could increase the expression of SOD, GSH activity and ATP expression, and reduce the content of MDA and NO in the colon mucosa of rats, but the expression level varied with dose. Conclusion: Kui Jie Shuang enema can significantly reduce the oxidative stress damage in the colon mucosa of UC model rats and im- prove the level of energy metabolism, thus reducing the damage of intestinal inflammation and promoting the repair of intestinal muco-sa.%目的:分别从氧化应激及能量代谢角度研究溃结爽灌肠方对实验性溃疡性结肠炎 (UC) 大鼠的作用及其疗效机制, 并应用正交试验设计, 分析溃结爽灌肠方中各组分在不同水平对UC大鼠的的单因素效应及量效关系.方法:按照L9(34) 正交表设计, 70只清洁级雄性wistar大鼠进行9组试验.葡聚糖硫酸钠 ( DSS) 法复制UC大鼠模型后, 以溃结爽方灌肠10 d, 麻醉取材, 计算结肠黏膜损伤评分 (CMDI), 制作病理切片; 生化法检测大鼠结肠黏膜谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD) 活性及丙二醛 (MDA)、腺苷三磷酸 (ATP)、一氧化氮 (NO) 表达水平.结果:模型组抗氧化因子SOD、 GSH活性明显降低, 脂质过氧化产物MDA及NO水平显著增高, 并且能量代谢受到影响, ATP水平降低, 与空白组比差异有显著性 ( P <0. 05).溃结爽灌肠能够不同程度改善UC大鼠氧化应激损伤及能量代谢水平, 提高SOD、 GSH活性及ATP水平, 降低MDA、 NO的表达, 并且以B组效应最佳 ( P <0. 05).在结肠黏膜损伤评分 (CMDI) 方面, A组B组C组E组F组I组均较模型组有不同程度改善 ( P <0. 05), 结果与抗氧化因子活性 (SOD、 GSH) 及能量代谢 (ATP酶) 表达水平变化具有一致性. 4味中药黄柏、苦参、白及、地榆在高、中、低剂量均可不同程度提高抗氧化因子SOD、 GSH活性及ATP的表达, 同时降低大鼠结肠黏膜MDA、 NO的含量, 但随剂量变化其表达水平呈现不同变化趋势.结论:溃结爽灌肠方可以明显降低UC模型大鼠结肠黏膜氧化应激损伤, 提高能量代谢水平, 从而减轻UC大鼠肠道炎症损伤, 促进肠道黏膜修复.
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