转录组学研究群体感应系统在生物被膜中的独特表达

摘要

Objective To study the unique expression of the quorum sensing in the bacteria biofilm formation.Methods We selected multidrug resistant Acinetobacter baumannii strains.Then we compared the two genomes in the rapid growth phase and biofilm state.Results In the same cell,the expressions of genes in the rapid growth phase and biofilm stage were different.The most significant factors in the expression of genes were the outer membrane proteins and the regulation of intracellular pathways.In the biofilm state,these regulators played a crucial role in the formation and maintains.Among them,the quorum sensing was one of the most important regulatory factors.It was significantly increased in the biofilm state.Conclusion The formation of bacteria biofilm could cause the long term survival of Acinetobacter baumannii.In the biofilm,the bacterial strain was continuously protected,resulting in increased resistance to the strain and repeated chronic infection in the hospital.The formation and morphology of the biofilm were relied on the regulation of quorum sensing.It may be involved in the regulation of biological behavior and contact among colonies in biofilms,so that the biofilm can be maintained as a whole.%目的 研究群体感应系统基因簇在菌体生物被膜状态的独特表达.方法 收集河北医科大学第二医院临床检验科细菌室,分离培养的多重耐药鲍曼不动杆菌;比较同一菌株在不同生长状态,即快速生长期和生物被膜状态下,群体感应系统基因簇的不同表达.结果 同一菌体在快速生长期和生物被膜包埋期有着显著不同的基因表达,表达差异基因最显著的集中于菌体外膜蛋白的表达和细胞内通路的因子调控.菌株在生物被膜状态下,表达差异的基因在生物被膜形成和形态维持过程中,起到了至关重要的作用.其中,群体感应系统基因簇是重要的调控因子之一.在细菌生物被膜状态下,表达显著升高.结论 细菌生物被膜的形成,可以使鲍曼不动杆菌长期存活在生物或非生物的表面;在生物被膜中,细菌菌株得到持续的保护,造成了菌株耐药性的增加和院内感染的反复发生.菌体生物被膜的生成和形态维持,其中很重要的调控因子是群体感应系统基因簇.它可能参与调控生物被膜内各个菌落间生物行为和联系,使得生物被膜整体维持平衡.