肝癌患者血清中miR-202表达量与肿瘤标志物含量、癌基因表达量的相关性研究

摘要

Objective:To study the correlation of serum miR-202 expression with tumor marker levels and oncogene ex-pression in patients with liver cancer.Methods:A total of 58 patients with liver cancer who received surgical resection in Xian-gyang Central Hospital Affiliated to Hubei University of Arts and Science between July 2014 and October 2016 were selected as the liver cancer group of the research,and 42 healthy volunteers who received physical examination during the same period were selected as the control group of the research.Peripheral blood was collected to determine the expression of miR-202 and the levels of tumor markers;liver cancer tissue and adjacent tissue were collected to determine the mRNA expression of prolif-eration genes and invasion genes.Results:Serum miR-202 expression in liver cancer group was significantly lower than that in control group(P<0.05);serum GP73,GPC3,AFP and AFP-L3 levels in liver cancer group were significantly higher than those in control group(P<0.05)and negatively correlated with serum miR-202 expression;LETM1,PI3K,AKT,FOXQ1, cyclinD1,c-myc,IFITM3,MMP9,Snail,N-cadherin and Vimentin mRNA expression in liver cancer tissue were significantly higher than those in adjacent tissue(P<0.05)and negatively correlated with serum miR-202 expression.Conclusion:The low-er serum miR-202 expression in patients with liver cancer is closely related to the abnormal proliferation and invasion of cancer cells.%目的:研究肝癌患者血清中miR-202表达量与肿瘤标志物含量、癌基因表达量的相关性.方法:选择在湖北文理学院附属襄阳市中心医院接受手术切除治疗的58例肝癌患者作为研究的肝癌组,同期体检的42例健康志愿者作为研究的对照组.采集外周血并检测miR-202的表达量及肿瘤标志物的含量;采集肝癌组织和癌旁组织并检测增殖基因、侵袭基因的m RN A表达量.结果:肝癌组患者血清中miR-202的表达量显著低于对照组;肝癌组患者血清中GP73、GPC3、AFP、AFP-L3的含量显著高于对照组且与血清中miR-202的表达量呈负相关;肝癌组织中LETM1、PI3K、AKT、FOXQ1、cyclinD1、c-myc、IFITM3、MMP9、Snail、N-cadherin、Vimentin 的 mRNA 表达量显著高于癌旁组织且与血清中miR-202的表达量呈负相关.结论:肝癌患者血清中miR-202的低表达与癌细胞的异常增殖和侵袭密切相关.