洛伐他汀对抗AβOs引起的SH-SY5Y细胞毒性作用

摘要

目的:观察洛伐他汀对抗Aβ寡聚体(AβOs)引起的SH-SY5Y细胞毒性作用.方法:生长至80％～90％并用无血清培养基培养12 h的SH-SY5Y细胞分为对照组、洛伐他汀处理组(0.1μmol/L洛伐他汀处理24h)、AβOs处理组(0.5μmol/L AβOs处理48 h)和洛伐他汀加AβOs处理组(0.1μmol/L洛伐他汀处理24h,再加入0.5μmol/L AβOs继续处理48 h),采用CCK-8法检测各组细胞活性,使用相应的试剂盒检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量.结果:0.5 μmol/L AβOs处理SH-SY5Y细胞48 h后,与对照组相比,SH-SY5Y细胞活性、SOD及GSH-Px活性显著降低,MDA含量明显增加(P＜0.05);在0.5 μmol/L ApOs处理SH-SY5Y细胞前,预先用0.1μmol/L洛伐他汀处理细胞24 h,可减弱AβOs引起的细胞活性、SOD和GSH-Px活性及MDA含量的改变(P＜0.05).结论:洛伐他汀可对抗AβOs引起的SH-SY5Y细胞毒性作用,其保护机制可能与抑制氧化应激有关.%Objective:To investigate the effect of lovastatin on cytotoxicity in SH-SY5Y cells induced by β-Amyloid peptide oligomers (AβOs).Methods:When SH-SY5Y cells were grown to 80％ ～90％ and cultured in serum-free medium for 12 h,they were divided into control group,lovastatin group (0.1 μmol/L lovastatin for 24 h),AβOs group (0.5 μmol/L βOs for 48 h) and lovastatin +AβOs group (0.1 pμmol/L lovastatin treated with for 24 h,and then treated with 0.5 μmol/L AβOs for 48 h).The cell viability was measured by CCK-8 assay,superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities and malondialdehyde (MDA) content were measured by appropriate biochemical methods.Results:As compared with control group,the cell viability,SOD and GSH-PX activities were significant decreased,while MDA content was significantly increased after SHSY5Y cells treated with 0.5 μmol/L AβOs for 48 h (P ＜0.05).When SH-SY5Y cells were treated with 0.1 μmol/L lovastatin for 24 h before treatments of AβOs,these cytotoxicity resulted by AβOs indicated as cell viability,SOD and GSH-PX activity and MDA content were all significantly attenuated (P ＜0.05).Conclusions:Lovastatin could antagonize the cytotoxicity in SH-SY5Y cells caused by AβOs,and the mechanism might relate to the inhibition of oxidative stress.