牛磺酸对锰致大鼠空间记忆能力衰退的影响

摘要

Objective:To investigate the effect of taurine in improving the spatial memory ability of rats that affected by manganese.Methods:Forty-two healthy SD male rats were chosen and randomly divided into control group (group C),manganese group (group M),and taurine group (group T) with 14 in each group.Rats in groups M and T were injected intra-peritoneally with manganese chloride solution 15 mg/(kg · d) or taurine 200 mg/(kg · d) respectively,five days a week for 3 weeks,while rats in group C were injected with 0.9 NaCl solution 30mg/(kg · d) instead for the same course.Morris water maze test and diving tower test were performed to evaluate special memory ability.Finally,hippocampus of rats were taken out and glutamine-related enzymes and MDA,gsh-px and SOD levels were detected.Results:Compared with those in group C,the escape latency and swimming distance in group M were significantly longer (P ＜ 0.05),and the glutamine synthetase,glutaminase,glutamic acid decarboxylase in group M decreased significantly (P ＜ 0.05).In group M,MDA contents and GSH-Px activity were higher and SOD content was lower than those in group C significantly (P ＜ 0.05).Compared with those in group M,escape latency and swimming total distance in group T were obviously shorter (P ＜ 0.05).The glutamine synthetase,glutaminase,glutamic acid decarboxylase and SOD content in group T were significantly higher than those in group M (P ＜ 0.05),while the MDA content and GSH-Px activity of group T was obviously lower than those in group M.Conclusion:Taurine could reduce damage in nerve cells of rats,improve spatial memory and reduce oxidative damage.%目的:探讨牛磺酸对锰致大鼠空间记忆能力衰退的改善作用.方法:选取健康SD雄性大鼠42只,随机均分为对照组、锰组、牛磺酸组;锰组和牛磺酸组腹腔注射氯化锰溶液15 mg/(kg·d),每周5d,共3周,牛磺酸组同期皮下注射牛磺酸溶液200 mg/(kg·d)每周5d,共3周,对照组同期腹腔注射0.9％ NaCl溶液30 mg/(kg·d);Morris水迷宫记录各组大鼠的空间探索时间及搜索次数,以评价大鼠的空间记忆能力;行为学实验后取各组大鼠海马,检测谷氨酸相关酶、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及超氧化物歧化酶(SOD)水平.结果:与对照组相比,锰组逃避潜伏期、游泳总路程均明显延长(P＜0.05),谷氨酰胺合成酶、谷氨酰胺酶、谷氨酸脱羧酶明显减少(P＜0.05),MDA含量、GSH-Px活性明显增加,SOD含量明显减少(P＜0.05);与锰组相比,牛磺酸组逃避潜伏期、游泳总路程均明显缩短(P＜0.05),谷氨酰胺合成酶、谷氨酰胺酶、谷氨酸脱羧酶明显增多(P＜0.05),MDA含量、GSH-Px活性明显减少,SOD含量明显增加(P＜0.05).结论:牛磺酸减少锰致大鼠的神经细胞损害,改善了空间记忆能力,减少了氧化损伤.