上海市水产品中副溶血性弧菌的分离、鉴定及毒力基因和血清型分布

摘要

Vibrio parahaemolyticus (Vp) has been considered as one of the most threatening foodborne bacterial pathogens.The aim of this study is to uncover the situation of contamination of Vibrio parahaemolyticus which isolated from the shellfish in the retail market of Shanghai,and provide some guidelines for Vp prevention.For this, we carried out the international method GB/T 4789.7-2008 to isolate bacteria and analyze its physiological and biochemical characteristics.Additionally, some primers for tlh specific genes and virulence genes (tdh, trh) recommended by FDA were used for the PCR analyze.The result shows that 45 strains of Vibrio parahaemolyticus were isolated from 161 samples which come from the shellfish market of Shanghai The average detection rate is 27.95％ Based on the PCR detection of tdh and trh gene,there were only 7 tdh gene found among the 45 strains, but none of them contain the trh gene.45 strains of Vp consisted of 7 serum groups:O1 2.2％ (1/45), O3 24.4％ (11/45),O4 15.6％ (7/45),O5 20.0％(9/45),O9 4.4％ (2/45),O10 8.9％(4/45),O11 26.7％(12/45).%为了了解上海市市售水产品中副溶血性弧菌(Vibrio parahaemoly ticus,Vp)污染状况和血清型,为防治Vp引起的食源性疾病提供依据.采用GB/T 4789.7-2008国标方法进行分离和生理生化鉴定,根据FDA推荐的引物对种特异性tlh基因和毒力基因(tdh,trh)进行PCR检测.从161份水产品中分离鉴定得到45株副溶血性弧茵,平均检出率为27.95%.通过PCR检测分离株的tdh和trh毒力基因发现,45株副溶血性弧菌均为trh阴性,7株为tdh阳性.45株副溶血性弧菌经血清凝集,结果共分出7个血清群,分别为O1群占2.2%(1/45),O3群占24.4%(11/45)、O4群占15.6%(7/45)、O5群占20.0%(9/45)、O9群占4.4%(2/45)、O10群占8.9%(4/45)、O11群占26.7%(12/45).