未培养微生物木聚糖基因真核表达及产酶条件优化

摘要

对来源于土壤宏基因组的未培养微生物筛选的木聚糖酶基因X1-19进行了毕赤酵母异源重组并对其产酶条件进行了优化.最佳的甲醇添加终体积分数为1％,接种密度为8x108个/mL,YNB的质量分数为1.2％,240 r/min,初始pH为5时,转化子产酶能力最高为(79.0±2.2) U/mL.研究为未培养微生物木聚糖酶基因的异源表达工业生产提供理论依据,具有较好的工业应用前景.%The non-cultured microbial xylanase gene X1-19 coming from metagenomics had been expressed on this paper by using Pichia pastoris as recombinant vector.The enzyme production conditions had been optimized.The results were shown as follows:the optimal methanol added final concentration was 1％,inoculum size was 8x108 / mL,the concentration of YNB was 1.2％,the initial pH value was 5,respectively.The highest enzyme yield was (79.0±2.2) U/mL.This research provided the theoretical basis for the heterologous expression of the non-cultured microbial xylanase gene,and had good prospects for industrial application.