基于国标法和试剂盒法对乳粉质控样品生物素含量的测定

摘要

Objective The biotin content in quality control milk powder was determined by national standard method and kit method, and the differences of results by two methods were evaluated.Methods Lactobacillus plantarum had the characteristics of sensitivity and specificity to the free biotin, it could reflect the biotin content in milk powder by measuring the growth of the strain.ResultsThe results showed that the interrelating coefficient of the two methods were good, the standard curve of national standard method was in the range of 0~1 ng/100 g(R2=0.9993) and the kit method was 0~0.72μg/100 g(R2=0.9983). The content of biotin that tested by two methods had no significant difference(P>0.05), and they all conformed to the requirements of the test, which were with the scope of the limited range.ConclusionIt is feasible to detect the biotin in milk powder by national standard method and kit method for its high accuracy.%目的：用国标法和试剂盒法对乳粉质控样品的生物素含量进行测定，评价两种方法对生物素检测结果差异性。方法根据两种方法中植物乳杆菌(Lactobacillus plantarum)对游离生物素的特异性和灵敏性，测定菌株的生长情况从而得到乳粉中生物素的含量。结果国标法测定所得标准曲线范围为0~1 ng/100 g(R2=0.9993)，试剂盒法测定所得的标准曲线范围是0~0.72μg/100 g(R2=0.9983)，线性关系良好；两种方法测定生物素的含量差异不显著(P＞0.05)，结果均符合要求并在给定值的范围内。结论两种方法对质控样品检测结果准确，稳定性好，适用于乳粉中生物素的检测。