高效液相色谱法检测电子束辐照水产品中的游离邻酪氨酸

摘要

Objective To establish a method for the determination ofo-tyrosine in irradiated seafood by high performance liquid chromatography (HPLC).MethodsThe sample was homogenized with 0.1% formic acid, the ultrasonic bath and centrifugation were followed. Then the resulting supernatants were treated with acetone at20℃ for 2 h and centrifuged again. After evaporation of acetone from supernatants, the remaining solution was dried freeze. Finally, the powder was collected, reconstituted in formic acid and detected by HPLC analysis with external reference method. For HPLC separation, Aglient ZORBAX Eclipse-C18 column was used with a flow rate at 0.8 mL/min, mobile phase: methanol-0.1% formic acid in Milli-Q water, and the fluorescence detector was set atλex275 nm andλem305 nm.ResultsThe recoveries ofo-tyrosine at the spiked amounts of 50, 200, and 500μg/kg were 72.20%~85.68% with RSD value between 1.716% to 9.926%. ConclusionThis method can be applied to detecto-tyrosine in electron beam irradiated seafood. The content of dissociativeo-tyrosine in some seafood was metapenaeusensis>lateolabrax japonicas> scallop.%目的：建立辐照水产品中邻酪氨酸的高效液相色谱检测方法。方法样品经0.1%甲酸均质,超声促溶后,离心去除不溶物质,加丙酮沉淀多余蛋白后,挥发有机试剂,冻干后复溶,高效液相色谱检测,外标法定量。采用Aglient ZORBAX Eclipse-C18色谱柱,甲醇-0.1%甲酸为流动相,激发波长为275 nm,发射波长为305 nm,流速为0.8 mL/min。结果样品在50、200、500µg/kg添加水平的平均回收率72.20%~85.68%,相对标准偏差(RSD)为1.716%~9.926%。结论该方法可用于电子束辐照水产品中邻酪氨酸含量的检测,不同种类水产品中游离酪氨酸含量由高到低依次为基围虾>鲈鱼>扇贝。