云南产6种蜂蜜中35种游离氨基酸的测定

摘要

Objective To establish a method for detection of 35 kinds of free amino acids by amino acid analyzer. MethodsHoney was dissolved with water, and the quantitative detection was carried out by automatic amino acid analyzer. Proline was detected by fluorescence detector at the wavelength of 440 nm while the other 34 kinds of amino acid were detected at the wavelength of 570 nm.ResultsHoney contained rich free amino acid compositions, and the composition and content of amino acids existed big differences. The main amino acid in honey was proline, and the content of proline was 0.042978 g/100 g, accounting for 71.21% of the total content of amino acids. Linear regression coefficients of 35 kinds of free amino acids were all above 0.99, and the recoveries were in the range of 92.4%~109.4%.Conclusion The method is rapid and accurate, which can be used for the determination of free amino acids in honey.%目的：采用全自动氨基酸分析仪测定蜂蜜中35种游离氨基酸的含量。方法蜂蜜样品经水溶解,采用全自动氨基酸分析仪进行定量检测。检测器为荧光检测器,脯氨酸在波长440 nm处测定,其余34种氨基酸在570 nm波长下测定。结果蜂蜜中含有丰富的游离氨基酸成分,且氨基酸组成及含量存在较大区别。脯氨酸是蜂蜜中的主要氨基酸,其平均含量为0.042978 g/100 g,占氨基酸组分总含量的71.21%。35种游离氨基酸的线性相关系数均达到0.99以上,回收率在92.4%~109.4%范围内。结论该方法快速、准确,可用于蜂蜜中游离氨基酸含量的测定。