葛根发酵液对原发性高血压大鼠的降血压作用

摘要

目的：分析葛根发酵液对原发性高血压大鼠(spontaneously hypertensive rat, SHR)的降血压作用。方法利用植物乳杆菌发酵葛根汁得到葛根发酵液,将 SHR 大鼠随机分为6组,空白对照组、依那普利组、葛根汁组、葛根发酵液低、中、高剂量组。通过不同剂量的单次灌胃和5周连续灌胃给药实验,测定受试动物的血压情况及血液中一氧化氮(NO)和超氧化物歧化酶(SOD)的含量,分析葛根发酵液的降血压能力。结果与空白对照组和葛根汁组相比,葛根发酵液低、中、高剂量组的SHR大鼠的血压显著降低(P<0.05),且在同等灌胃剂量下,葛根发酵液的降血压能力显著优于葛根汁。血液中 NO 和 SOD 含量的测定结果表明,中、高剂量的葛根发酵液能够显著提高SHR大鼠血清中NO和SOD的含量(P<0.05),而葛根汁组、低剂量组和依那普利组的大鼠血清中NO和SOD的含量升高不显著(P>0.05)。结论葛根发酵液对SHR大鼠具有良好的降血压作用。%Objective To analyze the effects of puerarin fermented liquid (PFL) on reducing blood pressure of spontaneously hypertensive rats (SHR).Methods The puerarin juice was fermented withLactobacillus plantarum to obtain the PFL. The SHR were randomly divided into 6 groups, which included control group, enalaprilat group, puerarin juice group, low, middle and high dose group of PFL. Through the single and long-term (5 weeks) lavage experiments, the antihypertensive effects of PFL were analyzed by determining the changes of blood pressure and content of nitric oxide (NO) and superoxide dismutase (SOD) in blood of SHR.ResultsCompared with control group and puerarin juice group, the blood pressures of SHR treated with low, middle and high dose of PFL were significantly reduced (P<0.05), and under the same lavage dose, the ability of PFL to reduce blood pressure was significantly better than that of puerarin juice. The content of NO and SOD in blood of SHR showed that the serum NO and SOD content of SHR treated with middle and high dose of PFL were significantly increased (P<0.05), however, there was no significant increase of serum NO and SOD content of SHR treated with puerarin juice, low dose of PFL and enalaprilat (P>0.05).Conclusion The PFL has a good antihypertensive effect to SHR.