阿里红中多糖的分离纯化及其组分分析

摘要

目的：对阿里红中的多糖进行分离纯化，并对得到的两种多糖组分进行基础结构分析。方法阿里红经水提醇沉法除蛋白后，再经DEAE纤维素-52、Sepharose CL-6B和葡聚糖凝胶Sephadex G-100柱层析分离纯化得到两种多糖组分FOPS-a和FOPS-b。采用凝胶过滤法分析其纯度和相对分子量，经气相色谱法分析其单糖组成，并对多糖组分进行部分酸水解，高碘酸氧化和 Smith 降解分析。结果分离纯化得到的阿里红多糖组分FOPS-a、FOPS-b的相对分子质量为199 kDa和87 kDa。单糖组成均为甘露糖、阿拉伯糖、半乳糖。FOPS-a主链单糖残基为甘露糖，末端残基和分支单糖残基为阿拉伯糖、半乳糖、甘露糖和鼠李糖； FOPS-b主链单糖残基为甘露糖，末端残基和分支单糖残基为阿拉伯糖、半乳糖、甘露糖和葡萄糖。结论该研究可为阿里红多糖的开发与利用提供技术参考。%Objective To isolate and purify two polysaccharides fractions fromFomes officinalis and clarify their basic chemical structures. Methods Two polysaccharides including FOPS-a and FOPS-b were obtained after extraction with boiling-water, precipitation with alcohol, and sequentially purification by DEAE cellulose-52 , Sepharose CL-6B and Sephadex G-100 gel column. Their homogeneity and relative molecular weights were analyzed by gel filtration and the monosaccharide compositiona were analyzed by gas chromatograph (GC). Partial hydrolysis with acid, periodate oxidation and Smith degradation were used to analyze their chemical structures. Results The molecular weights of FOPS-a and FOPS-b were respectively 199 kDa, 87 kDa. They were composed ofmannose, arabinose, and galactose. The main chain of FOPS-a was mainly made up of mannose, and the side chain was composed of arabinose, galactose, mannose,and xylopyranose. The main chain of FOPS-b was mainly made up of mannose, and the side chain was composed ofarabinose, galactose, mannose,andglucose. Conclusion This research can provide technical references for the development and utilization of polydaccharides ofFomes officinalis.