芎芷地龙汤不同时间预防给药对偏头痛动物模型PKCγ、PKCε mRNA的影响

摘要

Objective:To study the effect of Xiongzhi Dilong Decoction predministration on PKCγ and PKCε mRNA in migraine animal at different time.Methods:36 healthy male SD rats were randomly divided into six groups:saline group (n =6),migraine model group (n =6),sumatriptan group (n =6),1 day preadministration group (n =6),3 day preadministration group (n =6),7 day preadministration group (n =6).The drug was administered.Tissues were collected 2 h after successful modeling.The expression of PKCγand PKCε mRNA in trigeminal ganglion,spinal trigeminal nucleus,thalamus and dura mater were assayed with real-time PCR method.Resuits:Compared with saline group,the expression of PKCγ mRNA in the model group increased significantly in trigeminal ganglion,spinal trigeminal nucleus and dura mater,and decreased significantly in thalamus (P＜ 0.01).After drug intervention,the expression of PKCγmRNA decreased in trigeminal ganglion,spinal trigemina1 nucleus and dura mater,and the preadministration of 7 days group and sumatriptan group decreased significantly (P＜0.01).The expression of PKCγ mRNA increased in thalamus,and the preadministration of 1days group (P ＜0.01),3 days group (P＜0.05) and sumatriptan group (P＜ 0.01) increased significantly.Compared with saline group,the expression of PKCε mRNA in the model group increased significantly in trigeminal ganglion,spinal trigeminal nucleus and dura mater(P＜ 0.01),and decreased significantly in thalamus (P＜ 0.01).After drug intervention,the expression of PKCε mRNA decreased in trigeminal ganglion and spinal trigeminal nucleus,and the preadministration of 7days group and sumatriptan group decreased significantly(P ＜ 0.01).The expression of PKCε mRNA decreased in dura mater,and the preadministration of 3days group (P＜ 0.01) and sumatriptan group (P＜ 0.01) decreased more significantly than preadministration ldays group (P＜ 0.05) and 7 days group (P＜ 0.05).The expression of PKCε mRNA increased in thalamus,and the increase of all groups was statistically significant (P＜0.01).Conclusion:Preadministration of Xiongzhi Dilong Decoction can reduce the expression of PKCγmRNA and PKCε mRNA in trigeminal ganglion,spinal trigeminal nucleus and dura mater which are induced by NTG.The prevention of 7days is better than that of 3days and 1days.%目的 观察芎芷地龙汤不同时间预给药对偏头痛动物模型PKCγ、PKCε mRNA表达的影响.方法 将健康雄性SD大鼠36只,随机分为生理盐水组、模型组、舒马普坦组、芎芷地龙汤1d、3d、7d预给药组,每组6只.按照预定给药,造模结束后2h取材,real-time PCR法测定三叉神经节、三叉神经脊束核丘脑、硬脑膜PKCγ、PKCε mRNA表达情况.结果 与生理盐水组比较,模型组PKCγ mRNA在三叉神经节、三叉神经脊束核、硬脑膜表达水平明显升高,在丘脑表达水平明显降低(P＜0.01),给药干预后,三叉神经节、三叉神经脊束核、硬脑膜PKCγ mRNA表达水平均降低,其中以预防给药7d组和舒马普坦组降低明显(P＜0.01);丘脑PKCγ mRNA表达水平有所升高,以1d组、3d组、舒马普坦组明显((P＜0.05或P＜0.01).与生理盐水组比较,模型组PKCε mRNA在硬脑膜、三叉神经节、三叉神经脊束核表达水平明显升高(P＜0.01),在丘脑表达水平降低(P＜0.01);给药干预后,PKCe mRNA在三叉神经节、三叉神经脊束核表达水平降低,以预防给药7d组和舒马普坦组降低明显(P＜0.01),在硬脑膜表达水平降低,以3d组和舒马普坦组(均P＜0.01)较1d组和7d组(均P＜0.05)明显;PKCε mRNA在丘脑表达水平升高,各组升高水平均有统计学意义(P＜0.01).结论 芎芷地龙汤预防给药可以减少NTG诱发的三叉神经节、三叉神经脊束核PKCγ mRNA、PKCε mRNA表达,而以预防给药7d效果好于预防给药3d、1d.