芎芷地龙汤不同时间预防给药对偏头痛动物模型iNOS mRNA、c-fos mRNA表达的影响

摘要

Objective:To study the effect of Xiongzhi Dilong decoction preadministration at different time on iNOS mRNA,c-fos mRNA expression in migraine animal.Methods:36 healthy male SD rats were randomly divided into six groups:saline group (n =6),migraine model group (n =6),sumatriptan group (n =6),1 day preadministration group (n =6),3 day preadministration group (n =6),7 day preadministration group (n =6).The drug was administered according to different groups,tissues were collected after subcutaneous injection of corresponding drug given 2h later.The c-fos mRNA,iNOS mRNA expression in trigeminal ganglion,spinal trigeminal nucleus,thalamus,dura mater was assayed by real-time PCR method.Results:Compared with saline group,the iNOS mRNA expression in trigeminal ganglion,spinal trigeminal nucleus,dura mater increased significantly,and the iNOS mRNA expression in thalamus decreased significantly in the model group (P＜ 0.01);after the drug intervention,the iNOS mRNA expression in trigeminal ganglion,spinal trigeminal nucleus,dura mater was decreased,and the preadministration of 7days group and sumatriptan group decreased significantly (P＜ 0.01).The iNOS mRNA expression in thalamus was increased,but there were no statistical significance compared with the model group (P＞ 0.05).Compared with saline group,the c-fos mRNA expression increased significantly in trigeminal ganglion(P＜ 0.01),and spinal trigeminal nucleus (P＜ 0.05),and the c-fos mRNA expression decreased significantly in thalamus (P＜0.05) and dura mater (P＜0.01);after the drug intervention,the c-fos mRNA expression in trigeminal ganglion,spinal trigeminal nucleus was decreased,and the preadministration of 7days group and sumatriptan group decreased significantly(P＜0.01).The c-fos mRNA expression in thalamus and dura mater was increased,and the results in the preadministration of 7days group and sumatriptan group were statistically significant (P ＜ 0.05).Conclusion:Preadministration of Xiongzhi Dilong decoction can reduce the c-fos mRNA,iNOS mRNA expression in trigeminal ganglion,spinal trigeminal nucleus which were induced by NTG,and can reduce the iNOS mRNA expression in dura mater.The prevention of preadministration of 7 days is better than the prevention of preadministration of 3 days and 1 day.%目的 观察芎芷地龙汤不同时问预给药对偏头痛动物模型iNOS mRNA、c-fos mRNA表达的影响.方法 将健康雄性SD大鼠36只,随机分为0.9％氯化钠注射液组(6只)、模型组(6只)、舒马普坦组(6只)、中药1d预给药组(6只),中药3d预给药组(6只)、中药7d预给药组(6只),按照预定给药,造模结束后2h取材,real-time PCR法测定三叉神经节、三叉神经脊束核丘脑、硬脑膜c-fos mRNA、iNOS mRNA表达情况.结果 与0.9％氯化钠注射液组比较,模型组iNOS mRNA在三叉神经节、三叉神经脊束核、硬脑膜表达水平明显升高,在丘脑表达水平明显降低(P＜0.01),给药干预后,三叉神经节、三叉神经脊束核、硬脑膜iNOSmRNA表达水平均降低,其中以预防给药7日组和舒马普坦组明显降低(P＜0.01);丘脑iNOS mRNA表达水平有所升高,但各组与模型组差异均无统计学意义(P＞0.05).与0.9％氯化钠注射液组比较,模型组c-fos mRNA在三叉神经节、三叉神经脊束核表达水平明显升高,在丘脑、硬脑膜表达水平降低;给药干预后,c-fos mRNA在三叉神经节、三叉神经脊束核表达水平降低,以预防给药7d组和舒马普坦组降低明显(P＜0.05或P＜0.01);c-fos mRNA在丘脑、硬脑膜表达水平升高,其中以预防给药7d组和舒马普坦组升高幅度显著(P＜0.05).结论 芎芷地龙汤预防给药可以减少NTG诱发的三叉神经节、三叉神经脊束核c-fos mRNA、i-NOS mRNA表达,可以减少硬脑膜iNOS mRNA表达,而以预防给药7d效果好于预防给药3d、1d.