目的 研究呼吸道合胞病毒(RSV)感染对哮喘小鼠RANTES(regulated upon activation normal T cell expressed and secreted)生成的影响.方法 将BALB/c小鼠分为对照组、哮喘组、RSV感染组和RSV感染哮喘组4组,每组各8只.先采用卵清白蛋白致敏法制作小鼠哮喘模型,随后以RSV感染小鼠,RSV感染后24 h处死小鼠.检测小鼠支气管肺泡灌洗液(BALF)中RANTES及IFN-γ的浓度,并计数炎性细胞数.结果 RSV感染组和对照组,RSV感染哮喘组和哮喘组小鼠之间,BALF中的RANTES浓度差异均有统计学意义(P均<0.05);而对照组和哮喘组,RSV感染组和RSV感染哮喘组小鼠之间的RANTES浓度差异则无统计学意义(P均>0.05).各组小鼠BALF中的IFN-γ浓度差异无统计学意义.哮喘组、RSV感染组、RSV感染哮喘组小鼠BALF中的各种炎性细胞计数均较对照组升高,差异均有统计学意义(P<0.05),哮喘小鼠以嗜酸性粒细胞增高为主,而RSV感染小鼠以中性粒细胞为主.结论 感染RSV可使小鼠RANTES的表达增加,但哮喘未能使小鼠RANTES表达增加.提示RANTES增加可能与RSV感染有关,而与哮喘状态无关.%Objective To investigate the character difference and clinical meaning of distortion product otoa-coustic emissions (DPOAE) and auditory brainstem response (ABR) in hearing screening of infants with hyperbiliru-binemia. Methods DPOAE and ABR were recorded with Smart-EP and Smart-OAE in 197 (394 ears) infants with hyperbilirubinemia. Follow-up was undertaken in 3 or 4 months after birth. Results Of 197 (394 ears) newborns, none of them showed obvious manifestations of bilirubin encephalopathy. The incidence of DPOAE abnormality was 14.47% (57/394 ears) , whereas the incidence of ABR abnormality was 76.65% (302/394 ears). The level of serum bilirubin concentration was associated with abnormal rates of ABR (P < 0.01) , but not DPOAE. Potential high risk facts often exist in those with abnormal DPOAE. Of infants with severe ABR abnormality, around 40.7% had no improvement in 3 or 4 months after birth. Conclusions Neonatal hyperbilirubinemia without obvious manifestations of bilirubin encephalopathy shows high incidence of ABR abnormality. First ABR then DPOAE, or combination of ABR and DPOAE is recommended in hearing screening of neonates with hyperbilirubinemia.
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