载脂蛋白B基因EcoRI、XbaI及载脂蛋白AI基因-75 bp、+83 bp位点多态性与冠心病的关系

摘要

目的 分析载脂蛋白B(apoB)基因EcoRI、XbaI位点和载脂蛋白AI(apoAl)基因-75 bp、+83 bp位点多态性与新疆石河子地区汉族人冠心病的关系.方法 采用聚合酶链式反应-限制性片段长度多态性分析法检测204例冠心病患者和132例正常对照者的apoB基因EcoRI、XbaI位点和apoAI基因-75 bp、+83 bp位点多态性.结果 (1)冠心病组和对照组apoB基因EcoRl、Xbal位点和apoAI基因-75 bp、+83 bp位点基因型及等位基因频率与新疆石河子地区汉族人冠心病发病无关.(2)冠心病组和对照组各基因型组合的总体分布不同(X2:23.497,P=0.024),E+-/X+-/M1+-/M2++ 及 E+-/X1--/M2++/M2++基因型组合频率在冠心病组和对照组的比值(9.3% vs 3%)比较差异有显著性(P＜0.05).(3)冠心病组中E++/X+-/M1+-/M2+- 基因型组合的患者 apoB 水平高于冠心病组和对照组平均水平(P均＜0.01),apoAI/apoB水平低于冠心病组或对照组平均水平(P均＜0.01);冠心病组E+-/X--/M1+-/M2++ 基因型组合的患者apoB水平高于冠心病组和对照组平均水平(P均＜0.05),apoAI/apoB水平低于冠心病组或对照组平均水平(P均<0.05),且其TG、LDL-C高于对照组(P均<0.05).结论 apoB 基因EcoRl、Xbal位点和apoAI基因-75 bp、+83 bp位点基因型联合中,E++/X+-/M1+-/M2++ 及 E+--/X--/M1+-/M2++ 与新疆石河子地区汉族人冠心病相关,其中的机制可能与基因变异引起apoB、apoAI/apoB的变化继而引起血脂变化等有关.%ObjectiveTo explore the relationship between polymorphisms of EcoRI,XbaI of apolipoprotein B(apoB) gene and -75 bp, +83 bp of apolipoprotein AI(apoAI) gene and coronary heart disease(CHD) in Han Chinese of Shihezi, Xinjiang, China. Methods These polymorphisms were analyzed by PCR-restriction fragment length polymorphism(PCR-PFLP). 204 CHD patients and 132 normal people were involved. Results It indicates that the four polymorphisms are not related with CHD respectively ;both E++/X + -/M1+-/M2++ and E +-/X --/M1+-/M2++ are related with CHD respectively and significantly; E ++ /X +-/M1+-/M2++ in CHD group is associated with increased apoB (P ＜ 0.01 ) and decreased apoAl/apoB (P ＜0.01 )compared with CHD and control groups;E+-/X--/M1+-/M2++ in CHD group is associated with increased apoB( P ＜ 0.05 ) and decreased apoAI/apoB ( P ＜ 0. 05 ) compared with CHD and control groups,increased TG and LDL-C ( P ＜ 0. 05 ) compared with control group. Conclusion It is thought that combined polymorphisms of apoB and apoAI gene are related with CHD,and perhaps these phenomena are brought about through increased apoB and decreased apoAI/apoB which are related with disorder of lipids.