大鼠脊髓损伤后GDNFmRNA表达变化及意义

摘要

Objective　To investigate the gene expressing of GDNFmRNA after crushed spinal cord injury(SCI)in rats.Methods　The specimen of spinal cord were taken at different time after T13 segments of spinal cord were injured by modified Allen crush method(10g×7.5cm).The level of GDNFmRNA was observed and compared before and after SCI,using semi-quantitative RT-PCR method with β-Actin as a inner consult.Results　Much minim expression of GDNFmRNA was found in spinal cord in normal adult rats,but it increased 5 times at 24 hours after SCI,20 times at 72 hours,4 times at 7 days and still higher than normal level 10 days after SCI.Conclusion It should be one kind of self-protective behave models for spinal cord to express high standard GDNFmRNA in the early stage after SCI.It might require large amount of GDNF for neuron to be repaired GDNF should be supplied early when it was applied to treat SCI.%目的　探讨GDNFmRNA在大鼠脊髓损伤后表达变化及其意义。方法　改良Allen’s脊髓撞击(10g×7.5cm)致伤大鼠T1段脊髓，以β-Actin为内参照物，应用半定量RT-PCR方法，观察大鼠脊髓损伤前及损伤后不同时间GDNFmRNA表达变化。结果　GDNFmRNA在正常成年大鼠脊髓微量表达，脊髓损伤后24h表达增加5倍，72h增加20倍，7d增加4倍，10d仍高于正常水平。结论　脊髓损伤后早期GDNFmRNA表达增加，是神经元自我保护作用的一种表现；损伤神经元修复需要大量GDNF，应用GDNF治疗脊髓损伤时应早期用药。