EXPRESSION CHANGES OF MIR-210 AND ITS TARGET GENE EPHRINA3 IN ISCHEMIC CORTEX AFTER STROKE IN ADULT RAT

摘要

The timely revascularization in ischemic region after stroke is crucial for recovery of neurological function but the regulatory mechanism of this process is still not clear. Mir - 210 is a hypoxia-specific microRNA, and evidence showed that overexpression of mir - 210 in endothelial cell response to hypoxia could stimulate endothelial cell migration and capillary formation by blocking its target gene ephrinA3 protein expression. Whether mir - 210 is involved in the regulation of angiogenesis after brain ischemia has not been reported yet. In this study, we investigated the expression changes of mir - 210 and ephrinA3 in adult rat ischemic brain cortex after stroke. Results showed that mir- 210 expression was significantly upregulated in ischemic cortex at 1,3 and 7 days after stroke; furthermore, a gradually decreased ephrinA3 protein level was simultaneously detected in ischemic cortex at 1, 3 and 7 days post-ischemia. We concluded that the hypoxic conditions in ischemic cortex could up or downregulate mir- 210 and ephrinA3 expression respectively, suggesting a potential role of mir- 210 in ischemia-induced angiogenesis after stroke.