丹参素对高糖刺激腹膜间皮细胞分泌纤维连接蛋白和I型胶原的影响

摘要

Objective To investigate the possible role of Danshensu on fibronectin ( FN ) and collagen-I (Col-I) secretion induced by high glucose in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were treated with high glucose and Danshensu at different concentrations. The mRNA expression of FN, Col-I, endothelin-I ( ET-1 ) , and heme oxygenase-1 ( HO-1 )were examined by reverse transcription-polymerase chain reaction ( RT-PCR ). The protein expression of FN, Col-I HO-1 and ET-1 were analyzed by enzyse-linked immunosorbent assay or immunofluorescence method. Results The expression of protein and mRNA of FN and Col-I were attenuated by Danshensu in both dose-dependent and time-dependent manner. The mRNA and protein levels of ET-1 were decreased, and the mRNA and protein levels of HO-I increased in the Danshensu groups in a dose-dependent manner compared with the high glucose group. The expression of ET-1 and HO-1 showed little difference in a time gradient of danshensu (P ＞ 0.05 ). Conclusion Danshensu can protect HPMCs through inhibiting the expression of FN and Col-I induced by high glucose, which is related to the suppression of oxidative stress.%目的:探讨丹参素对高糖刺激培养的腹膜间皮细胞(HPMCs) 分泌纤维连接蛋白(FN)和I型胶原(Col-I)的影响.方法:以高糖刺激培养HPMCs,分别加用浓度为10,5,2.5,1.25,0.625 mg/L的丹参素进行干预,并选取浓度为10 mg/L的丹参素干预0,12,24,48,72 h;应用RT-PCR,ELISA法检测FN和Col-I蛋白及mRNA表达;RT-PCR、免疫荧光法检测血红素氧化酶-1(HO-1),内皮素-1(ET-1)蛋白及mRNA表达.结果:丹参素可显著降低高糖诱导的HPMCs细胞外基质FN和Col-I蛋白及mRNA表达,呈浓度依赖性及时间依赖性;丹参素可抑制高糖诱导的ET-1蛋白及mRNA表达,增加诱导型抗氧化酶HO-1蛋白及mRNA表达,呈浓度依赖性;丹参素不同时间组ET-1和HO-1蛋白及mRNA表达差异无统计学意义(P＞0.05).结论:丹参素可减少高糖诱导的腹膜间皮细胞 FN和Col-I的分泌,其作用机制可能与拮抗氧化应激状态有关.