限制性液体复苏联合血必净注射液对失血未控制性休克兔失血量及复苏液体量的影响

摘要

Objective:To explore the effects of limited fluid resuscitation combined with Xuebijing injection on the blood loss and resuscitative fluid in rabbits with uncontrolled hemorrhagic shock. Methods; The model of 24 New Zealand rabbits with uncontrolled hemorrhagic shock was established, and rabbits were divided into XBJ group ( Xuebijing injection) , LRS group (lactated ringer' s solution) and NRC group ( none resuscitation control) (8 rabbits each group). The three groups were intervened with different resuscitative fluid in the limited and bleeding resuscitation phase. The number of survived rabbits,the blood loss,the volume of fluid input and hematocrit( HCT) changes of different time-phases of three groups were observed. Results; All animals in NRC group died at the end of the experiment( about 390 minutes) , and which average survival time was 152. 8 minutes. All animals in XBJ and LRS group survived. In the limited resuscitation phase, the blood loss in NRC group was the least. The blood loss in XBJ group was significantly less than that in LRS group ( P < 0. 01 ). In the limited and bleeding resuscitation phase, the resuscitative fluid in XBJ group was significantly less than that in LRS group( P < 0. 01). At the end of the limited and bleeding resuscitation phase and hospital observation period,the HCT in XBJ group was significantly higher than that in LRS group(P<0.01). Conclusions;Compared with LRS,the threatmentrnof rabbit with uncontrolled hemorrhagic shock with XBJ combined with LRS can reduce the amount of blood loss and resuscitative fluid,and decrease HCT in the limited fluid resuscitation phase, which is helpful for the recovery of animal shock.%目的:研究限制性液体复苏联合血必净注射液对失血未控制性休克兔失血量及复苏液体量的影响.方法:取24只新西兰兔,建立失血未控制性休克模型.随机分成3组,血必净组(XBJ组)、乳酸林格液组(LRS组)和不复苏组(NRC组),分别在限制复苏期和止血复苏期给予不同的复苏液体干预,观察兔子存活数量、失血量、补液量以及在各个时相点红细胞压积(HCT)的变化.结果:在实验结束(约390 min)时,NRC组全部死亡,平均存活时间152.8 min ;XBJ组和LRS组全部存活.在限制复苏期,NRC组失血量最少,XBJ组失血量明显少于LRS组(P＜0.01).在限制复苏期和止血复苏期,XBJ组的复苏液体量均少于LRS组(P＜0.01).在限制复苏期末(90 min)、止血复苏期末(210 min)和院内观察期末(390 min),XBJ组的HCT均高于LRS组(P＜0.01).结论:与乳酸林格液相比,血必净注射液联合乳酸林格液在限制性液体复苏时,能够减少失血未控制性休克兔的失血量、补液量和HCT的下降,有助于休克机体的复苏.