目的 对知母(Anemarrhena asphodeloide Bunge.)的干燥须根进行化学成分研究.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱及重结晶等手段进行分离纯化,运用波谱学方法鉴定化合物的结构.结果 从知母须根中分离得到15个化合物,分别鉴定为胡萝卜苷(1)、菝葜皂苷元(2)、芒果苷(3)、7-hydroxy-3-(4-methoxybenzyl)-chroman (4)、3′-hydroxy-4′-methoxy-4-dehydroxynyasol (5)、5,7-dihydroxy-3-(4-methoxybenzyl)-chroman-4-one(6)、2,4′-dihydroxy-4-methoxy benzophenone(7)、1,3-Bis(4-hydroxyphenyl) pent-4-en-1-one(8)、(+)-syringaresinol (9)、ent-16,17-dihydroxy-kauran-19-oic acid (10)、(+)-pinoresinol (11)、对羟基苯甲酸(12)、新芒果苷(13)、丁香酸(14)、4′,6-dihydroxy-4-met hoxy-benzophenone2-O-(2″),3-C-(1″)-1-desoxy-α-L-fructofuranoside (15).结论 化合物5～11、14、15为首次从知母须根中分离得到.%Objective To investigate the chemical constituents of the dried fibrous root of Anemarrhena asphodeloides.Methods Silica gel column chromatography,Sephadex LH-20 column chromatography,and recrystallization were used for isolation and purification,and spectroscopy was used to identify the structure of compounds.Results A total of 15 compounds were isolated from the fibrous root of Anemarrhena asphodeloides and were identified as daucosterol (1),sarsasaponin (2),mangiferin (3),7-hydroxy-3-(4-methoxybenzyl)-chroman (4),3′-hydroxy4′-methoxy-4-dehydroxynyasol (5),5,7-dihydroxy-3-(4-methoxybenzyl)-chroman-4-one (6),2,4′-dihydroxy-4-methoxy benzophenone (7),1,3-bis (4-hydroxyphenyl) pent-4-en-1-one (8),(+)-syringaresinol (9),ent-16,17-dihydroxy-kauran-19-oic acid (10),(+)-pinoresinol (11),4-hydroxybenzoic acid (12),neomangiferin (13),syringic acid (14),and 4,6-dihydroxy-4-methoxy-benzophenone-2-O-(2″),3-C-(1″)-1-desoxy-α-L-fructofuranoside (15).Conclusion Compounds 5-11,14,and 15 are obtained from the fibrous root of Anemarrhena asphodeloides for the first time.
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