阿维菌素高产工业菌株的推理选育研究

摘要

[Objective ] Strain Biok AV-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening. [ Method ] Using L-Ile as screening pressure after UV mutation for the strain. [ Result ] The result showed that the maximum aver-mectin production of a mutant stain AV6Gs-32 reached 4520 IU/ml,which was 23. 4% higher than the control. [Conclusion] This screening method of using the UV and L-L-Ile may be a simple and applicable way to avermectin industry.%[目的]通过推理选育,获得高产的阿维菌素工业生产菌种.[方法]以 Biok AV-023为出发菌株,首先采用常规紫外诱变筛选出正突变菌株,再通过添加L-异亮氨酸诱导推理选育方法选育高产阿维菌素生产菌株.[结果]经紫外诱变和L-lie定向筛选表明,L-lie筛选浓度以0.5％最佳,经复筛后获得的高产突变菌株AV60s-32最高效价可达4 520 IU/ml,与出发茵株相比提高了23.4％.[结论]采用紫外诱变和L-lie推理选育相结合的方法可有效提高阿维菌素菌种发酵效价.