首页> 中文期刊> 《安徽农业科学》 >致病疫霉拮抗细菌的筛选及抑菌作用研究

致病疫霉拮抗细菌的筛选及抑菌作用研究

         

摘要

[Objective] The paper was to screen bacterial strain with significant antagonistic effect against Phytophthora infestans, so as to provide basis for further development and utilization of antagonistic bacteria to control P. infestans and potato late bright. [ Method] Plant confrontation and filter paper method was used to determine the inhibition effect of living, fermentation product, and bacterial fluid of 61 strains a-gainst P. infestans and the induced resistance of SR13-2 strain. [Result] The inhibition rate of 24 strains among 61 tested strains against my-celial growth of P. infestans was greater than 60% , and the inhibition effect of HT-6 strain was the strongest with the inhibition rate of 89.92%. However, fermentation product of all tested strains had no significant inhibition effect on P. infestans, while the inhibition effect of bacterial fluid of most strains was significantly higher than living strain; the inhibition effect of S34-1 strain was the strongest with inhibition rate of 91.50%. The bacterial fluid of SR13-2 strain was found to have significant resistance-induction effect with protection rate of 60%. [Conclusion] The inhibition effect of living and fermentation product of antagonistic strains S34-1 and SR13-2 had no relationship with each other, while bacterial fluid had great potential in controlling potato late bright.%[目的]筛选获得显著拮抗致病疫霉的细菌菌株,为深入开发利用拮抗细菌抑制致病疫霉并控制马铃薯晚疫病提供依据.[方法]用平板对峙法和滤纸片法测定61株细菌活体、发酵液及茵液对致病疫霉的抑制作用并进一步测定了SR13-2菌株的诱导抗病作用.[结果]供试的61株细菌活体对致病疫霉菌丝生长的抑制率达到60%以上的有24株,其中HT-6菌株的抑茵作用最强,抑茵率达到了89.92%:但所有供试菌株的发酵液均无显著抑菌作用,而大部分菌株茵液的抑茵作用却明显高于活体菌株,其中以S34-1菌株的抑菌作用最强,抑菌率为91.50%;同时发现SR13-2菌株的菌液具有显著的诱导抗病作用,保护率可达60%.[结论]拮抗菌株S34-1和SR13-2的活体与发酵液的抑茵作用之间无相关性,而菌液在防治马铃薯晚疫病方面有较大的应用潜力.

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