HPLC法测定不同海拔地衣中松萝酸的含量

摘要

[目的]测定不同海拔地衣中松萝酸含量.[方法]以不同海拔的地农为材料,乙酸乙酯为溶剂,通过高效液相色谱法(HPLC)测定不同海拔的暗腹黄梅、淡腹黄梅、喇叭粉石蕊中松萝酸含量.[结果]淡膜黄梅中松萝酸含量最高,为0.789 1%,其次为暗腹黄梅,喇叭粉石蕊中最低.[结论]不同地衣体中松萝酸含量与海拔无明显关系,松萝酸含量与种间亲缘关系有关.%[Objective] To determine the content of usnic acid from lichens growing at different elevation. [Method] Using lichens at different elevation as materials and ethylacetate solvent, contents of usnic acid in Xanthoparmelia tinctina,Xanthoparmelia mexicanna and Cladonia chlorphaea were detennined by HPLC. [ Result ] The content of usnic acid in Xanthoparmelia mexicanna was highest, followed by Xanthoparmelia tinctina and Cladonia chlorpaea. [Conelusion ]Content of usnic acid in lichen which was chosely related with species had nothing to do with altiude.