[目的]筛选出长枣多糖中的抗肿瘤多糖,为长枣新产品的开发提供依据.[方法]对长枣中提取的6种多糖运用Sepharose CL-6B及高效液相色谱法分析检测,同时运用MTT法,对6种长枣多糖对人肝癌细胞株Bel7402、人胃癌细胞株BGC823、人鼻咽癌细胞株KB的增殖抑制率进行了测定,并进行了比较分析.[结果]检测结果表明,制得的6种长枣多糖均为单一组分多糖.当浓度为400 mg/L时,LJU-3对3种肿瘤细胞的抑制率分别为61.29％、68.77％、72.16％,均高于其他多糖,且高于50％,说明它对3种肿瘤的抑制作用为中度敏感或高敏感.运用IC50软件计算得它对Bel7402、BGC823、KB抑制的IC50值分别为198、178、167 mg/L,因此认为长枣多糖中抗肿瘤多糖为LJU-3.[结论]该研究为进一步深入研究枣多糖抗肿瘤作用机制奠定了良好的基础.%[ Objective] To filter the antitumor polysaccharide from long jujubes, so as to provide references for developing new jujube products. [ Method] Purity of the obtained six long jujube polysaccharides were detected with Sepharose CL-6B and HPLC. At the same time inhibition rate of Bel7402, BGC823 and KB by six long jujube polysaccharide were determined and comparatively analyzed. [ Result] The six types of polysaccharides were all single component. When the concentration was 400 mg/L, inhibition rate of the three tumour by LJU-3 were separately 61.29% , 68.77% and 72.16% ,which was the highest than that of other five long jujube polysaccharides and higher than 50% , all of this explained the inhibitive effect of LJU-3 against the three tumour was moderate sensitive or high sensitive. IC50 value of LJU-3 to Bel7402, BGC823 and KB were 198, 178 and 167 mg/L with IC50 software, so LJU-3 was considered as the antitumor polysaccharide in long jujube polysaccharide. [Conclusion] The study provided references for the furthur study on the antitumor activity of polysaccharides from long jujube.
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