卵丘细胞在牛卵母细胞体外受精生产胚胎中的作用

摘要

Bovine embryo in vitro production is an efficient approach to provide embryos for embryo transfer industry and it includes three procedures, in vitro maturation, in vitro fertilization (IVF) and in vitro culture.The qualities of embryos derived from IVF play an important role in successful rate of embryo transfer and live calves.Many researches reported that cumulus cells secret many important factors, such as steroids hormone and cell growth factors, contributed greatly to bovine oocytes in vitro maturation, fertilization and culture of fertilized oocytes.The current paper reviews recent advances about the effect of cumulus cells on bovine embryos produced by IVF, in which the effect of cumulus cells on oocyte in vitro maturation, the secreting function, quality, and cocultural system of cumulus cells on efficiency of IVF are highlighted and discussed.%牛体外胚胎生产是解决胚胎移植产业化胚胎来源的有效技术方法,其包括卵母细胞体成熟、体外受精和受精卵体外胚胎.而体外受精胚胎的质量是影响胚胎移植妊娠率与犊牛成活率的重要因素.体外受精过程中卵丘细胞分泌的一些重要物质(类固醇激素、细胞生长因子等),对牛卵母细胞体外成熟、体外受精以及受精卵体外发育都具有重要的促进作用.本文综合了近年来该领域的研究进展,着重分析了卵丘细胞对卵母细胞体外成熟,以及体外受精过程中卵丘细胞的分泌功能、质量和共培养体系对卵母细胞体外受精效率的影响.