为了进一步探索浙江省及周边地区猪圆环病毒2型(Porcine circovirus type 2,PCV2)的分子进化特征.本研究运用实验室已建立的PCR方法,采集2007～2012年期间浙江省及周边地区疑似PCV2感染的180份病例的肺脏、脾脏及淋巴结,并制成混合的组织样本,进行猪PCV2感染情况检测,并抽提组织细胞DNA作为模板,进行PCV2目的片段的全基因组扩增及测序,研究结果表明:浙江省及周边地区PCV2在2007～2012年阳性率分别为41.7％、52.0％、44.1％、37.0％、40.5％和33.3％,总阳性率41.4％.与以往相比,PCV2感染率基本保持一致.随机选取的45份阳性病料PCV2全基因组测序结果显示,45个毒株均属于Group 1(PCV2b),很显然,浙江省及周边地区PCV2的主导基因型依然是Groupl.45个毒株中属于1A分支的有11个毒株,属于1B分支的有10个毒株,属于1C分支的有22个毒株,所占比例分别是23.9％、21.7％和47.8％,同时发现随着PCV2在本地区的流行,1C分支从无到有,且在其中的比例也越来越高,并逐步占据主导地位.对PCV2毒株Cap变异的分析能更好掌握检测区域毒株同源性情况.%In order to investigate the molecular evolutionary characteristics of Porcine circovirus type 2 (PCV2) in Zhejiang and surrounding areas. One hundred and eighty field samples with mixed tissues(spleen, lung and lymph node) from suspect PCV2- infected pigs during 2007-2012 were detected for the PCV2 by the established PCR method, and T-A clone and sequencing were used to get the complete genome of PCV2. Seventy-four cases out of 180 diseased pigs from these herds were PCV2 positive. PCV2-positve rate between 2007 and 2012 (41.7%, 52.0%, 44.1%, 37.0%, 40.5% and 33.3%, respectively) remained in the same level that during 2004-2006. Forty-five PCV2 complete genomes belonged to subgroups 1A (23.9%), 1B (21.7%), 1C (47.8%) and Group 2 (0%), respectively.rnGroup 1 was still the dominant genotype of PCV2 in Zhejiang and surrounding areas. However, as the prevalence of PCV2 in this region, virus belonged to subgroup 1C appeared, increased and gradually dominated. The Cap variability of PCV2 isolates was analyzed in this study, and the result contributed to better grasp the homology of the strains in the detected area.
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