16种中药颗粒剂对5种耐药菌株的体外抑菌效果比较

摘要

Objective To explore the in vitro antibacterial effect of scutellaria baicalensis etc.16 kinds of Chinese herbal medicine granules on methicillin-resistant staphylococcus aureus (MRSA) etc.5 common clinical drug resistant bacteria.Methods Basing on the research on traditional Chinese medicine source,16 kinds of traditional Chinese herbal medicine granules with anti-inflammatory and antibacterial effects were chosen.K-B disc agar diffusion method was used to measure the size of inhibition ring of 5 kinds clinical common resistant bacteria.Meanwhile,a broad-spectrum antibiotic was selected as a positive control.t test was used to compare with the granule.Results Baicalin had best antibacterial effect; methicillin resistant Staphylococcus aureus was highly sensitive to it but lowly sensitive to the other 4 kinds; the diameter of inhibition zone was 6-19 mm.Forsythia and mint came behind it in inhibitory effect.Isatis root,honeysuckle,chrysanthemum,Tuckahoe,Folium Isatidis,Herba Violae,and Anemarrhena granules had no inhibitory effect on the five kinds of drug resistant bacteria.For methicillin-resistant Staphylococcus aureus (MRSA),Scutellaria baicalensis didn' t statistically differed from vancomycin (P ＞ 0.05),while the rest of the granuleswere worse than vancomycin (P ＜ 0.05).For resistant Pseudomonas aeruginosa,Fructus Forsythiae didn' t statistically differed from imipenem (P ＞ 0.05),while the rest of the granules were worse than imipenem (P ＜ 0.05).For the remaining 3 resistant bacilli,granules were worse than imipenem (P ＜ 0.05).Conclusion Part of the traditional Chinese medicine granules have inhibitory effect on the 5 clinical common resistant bacteria.%目的 探讨黄芩等16种中药颗粒剂对耐甲氧西林金黄色葡萄球菌(MRSA)等5种临床常见耐药菌的体外抑菌效果.方法 通过对中药药源的深入调查,选用16种具有消炎抗菌作用的中药颗粒剂,采用K-B纸片琼脂扩散法分别测量5种临床常见耐药菌的抑菌圈大小,同时各选用一种广谱抗生素作阳性对照,采用t检验与颗粒剂进行比较.结果 黄芩抑菌效果最好,耐甲氧西林金黄色葡萄球菌对其高度敏感,其他4种菌敏感度较低,抑菌圈直径在6～ 19mm之间;连翘、薄荷抑菌效果次之;板蓝根、金银花、菊花、土茯苓、大青叶、地丁、知母7种颗粒剂对这5种耐药菌没有抑菌效果;对于耐甲氧西林金黄色葡萄球菌(MRSA),黄芩与万古霉素比较差异无统计学意义(P＞0.05),其余颗粒剂均差于万古霉素(P＜0.05);对于耐药绿脓杆菌,连翘与亚胺培南比较差异无统计学意义(P＞0.05),其余颗粒剂均差于亚胺培南(P＜0.05);对剩下的3种耐药杆菌,颗粒剂均差于亚胺培南(P＜0.05).结论 部分中药颗粒剂对5种临床常见耐药菌有抑菌效果.