尿新蝶呤在112例儿童肺结核耐药性监测中的临床价值

摘要

目的 探讨尿新蝶呤在儿童肺结核耐药性监测中的临床价值.方法 应用纸片扩散法进行结核耐药性检测.根据耐药检测结果,将肺结核活动期患儿112例分为耐药组(n=48)和非耐药组(n=64),健康儿童50例作为健康对照组.ELISA法检测其尿新蝶呤水平.结果 结核耐药组、非耐药组、健康对照组尿新蝶呤水平差异有统计学意义,结核患儿尿新蝶呤水平显著高于健康对照组,耐药组又显著高于非耐药组.结论 尿新蝶呤水平测定不仅对儿童肺结核有辅助诊断价值,而且在儿童结核耐药性监测中有重要的预警价值.尿新蝶呤测定作为一种无创、快速、简捷、敏感的结核耐药性初筛方法,值得临床推广应用.%Objective To investigate the clinical value of urinary neopterin in monitoring of pulmonary tuberculosis drug resistance in 112 children.Methods Slip diffusion method was applied to performing tuberculosis drug resistance test. According to the detecting results, 112 children with active pulmonary tuberculosis were divided into resistance group(n=48) and non-resistance group(n=64). 50 healthy children were enrolled as healthy control group. ELISA was employed to measure the urinary neopterin level for all subjects.Results There were statistical differences in urinary neopterin levels among resistance group, non-resistance group and healthy control group. The urinary neopterin level was more elevated in patients with pulmonary tuberculosis than that of healthy controls;while, the urinary neopterin level was much higher in resistance group than that of non-resistance group.Conclusion Not only does the measurement of urinary neopterin play a assisted role in diagnosis of pulmonary tuberculosis in children, but also it is of prewarning value in monitoring of children pulmonary tuberculosis drug resistance. As a non-invasive, rapid, simple and sensitive preliminary screening index, urinary neopterin is worthy of spreading and applying in clinic.