重组腺相关病毒作为目标基因转移视网膜的载体的可行性研究

摘要

AIM:To study the feasibility of recombinant adeno-associated virus(rAAV)as a vector to transfer the green fluorescent protein(GFP)gene as a target gene into rabbit retina.METHODS:Intravitreal injection of rAAV-gfp was performed in either eye for each rabbit with the other eye taken as control.At the 3rd,7th,and 14th day after injection,the eyeballs were removed,and the retinas were flat-mounted on glass slides to inspect the retinal fluorescence,respectively.RESULTS:After intravitreal injection of rAAV-gfp,the presence of fluorescent spots in the cytoplasm of retinal cells indicated that GFP gene was efficiently transferred and expressed in the rabbit retina.CONCLUSION:Recombinant adeno-associated virus is a reliable and simple vector for transferring target gene,e.g.,GFP gene,to the retina.%目的:探讨重组腺相关病毒基因(recombinant adeno-associated virus gene,rAAV)载体转导绿荧光蛋白基因(green fluorescent protein,gfp)基因至视网膜的可行性.方法:18只家兔随机选取一眼玻璃体内注射rAAV-gfp,对侧眼作为对照.分别于注射后3,7,14d摘除眼球进行视网膜铺片观察视网膜的荧光.结果:家兔玻璃体注射rAAV-gfp后视网膜细胞浆内可见荧光点,提示gfp基因被有效转导至视网膜并进行荧光表达.结论:rAAV是一个可靠、简便易行的目标基因转移视网膜的载体.