目的 了解深圳市HIV分子流行病学及不同亚型混合感染情况.方法 对429例HIV-1感染者进行详细的流行病学调查,提取外周静脉抗凝血中的病毒核酸,RT-PCR巢式扩增病毒的包膜蛋白(env)和核心蛋白(gog)基因的核酸片段,结合HIV数据库和Mega软件分析测序结果,建立进化树,确定每个基因的亚型.结果 在400份合格序列的样本中有01-AE、07-BC、08-BC、02-AG 4种重组型和B、C2种亚型,01-AE是主要流行株;有6例样本的env和gag区基因分型结果存在不一致情况.结论 深圳市存在HIV-1多亚型共流行和混合感染情况.%Objective To investigate HIV -1 molecular epidemiology and co-infection of different subtypes in Shenzhen. Methods An epidemiological investigation was made in 429 cases of HIV-1 infection. Viral nucleic acid in the peripheral blood of the patients was extracted. RNA fragments of HIV-1 env and gag gene were amplified by nested reverse transcriptase-polymerase chain reaction. By analyzing sequencing results based on HIV database and Mega software, evolutionary tree was established to confirm the subtypes of each gene. Results In all of 400 qualified samples, there were 4 kinds of HIV-1 circulating recombinant forms (CRFs), 01-AE, 07-BC, 08-BC and 02-AG and 2 subtype strains, subtype B and C in the subjects in Shenzhen. CRF01-AE was the predominant strain. The subtypes of env and gag gene were not consistent in 6 samples. Conclusion The epidemic of HIV-1 subtypes and their co-infection emerge in Shenzhen.
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