鸭病毒性肝炎高免卵黄抗体研制与应用

摘要

用鸭肝炎病毒(DHV)地方分离毒株HB98株经鸡胚连续传53～62代适应鸡胚的鸭肝炎病毒作为种毒,制备成弱毒疫苗和灭活疫苗分别免疫伊莎产蛋鸡,经3次免疫后,收获卵黄,测定病毒中和效价可达103以上,经30万只雏鸭临床应用,对未发病的雏鸭预防保护率达90％以上,发病鸭群治疗保护率达80％以上.%A master seed was prepared by 53~62 serial passage of duck hepatitis virus (DHV) strain HB98 in chick embryo and used to develop attenuated vaccine and inactivated vaccine of DHV. The egg yolk was collected after 3 immunizations of Isa chick. The virus neutralizing titer tested was above 103. According to the clinical application on 300 000 ducklings, the prevention rate of egg yolk antibody for healthy duckling was above 90%; and the cure rate of diseased ducking was above80%